Elastase 3b (ELA3B) ELISA Kit for Human

Name: Human ELA3B ELISA Kit; Elastase 3b; MyBioSource
Brand: MyBioSource
SKU: MBS452221
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS452221

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Elastase 3B (ELA3B), ELISA Kit

Full Product Name

Human Elastase 3B (ELA3B) ELISA Kit

Product Gene Name

ELA3B elisa kit

Product Synonym Names

CELA3B; Chymotrypsin Like Elastase Family Member 3B; Protease E; Fecal Elastase; Elastase 1; Cholesterol-Binding Pancreatic Protease; Pancreatic Endopeptidase E

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma or other biological fluids

Detection Range

0.312-20ng/mL

Sensitivity

< 0.135ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of ELA3B. No significant cross-reactivity or interference between ELA3B and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELA3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELA3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

29,263 Da

3D Structure

ModBase 3D Structure for P08861

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ELA3B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELA3B purchase

MBS452221 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Elastase 3B (ELA3B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ELA3B. The ELISA analytical biochemical technique of the MBS452221 kit is based on ELA3B antibody-ELA3B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA3B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA3B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ELA3B. It may not necessarily be applicable to this product.

NCBI GI #

6679625

NCBI GeneID

23436

NCBI Accession #

NP_031378.1

UniProt Primary Accession #

P08861

UniProt Secondary Accession #

P11423; Q5VU28; Q5VU29; Q5VU30; B2RE44

UniProt Related Accession #

P08861

GenBank Nucleotide #

NM_007352.3

NCBI Official Full Name

chymotrypsin-like elastase family member 3B preproprotein

NCBI Official Synonym Full Names

chymotrypsin like elastase family member 3B

NCBI Official Symbol

CELA3B

NCBI Official Synonym Symbols

CBPP; ELA3B

UniProt Protein Name

Chymotrypsin-like elastase family member 3B

UniProt Synonym Protein Names

Elastase IIIB; Elastase-3B; Protease E

UniProt Gene Name

CELA3B

UniProt Synonym Gene Names

ELA3B

NCBI Protein Information

chymotrypsin-like elastase family member 3B

NCBI Summary for ELA3B

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]

UniProt Comments for ELA3B

Efficient protease with alanine specificity but only little elastolytic activity.

Research Articles on ELA3B

1. this study demonstrated a strong association of diabetes with low pancreatic elastase levels in feces

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ELA3B elisa kit

Reference Product	PubMed Publications
CELA2A elisa kit	>3 publications with ELA3B and CELA2A
CPA1 elisa kit	>2 publications with ELA3B and CPA1
CELA2B elisa kit	>1 publications with ELA3B and CELA2B
CPB1 elisa kit	>1 publications with ELA3B and CPB1

Pathways associated with ELA3B elisa kit

Products by Pathway	Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram

Diseases associated with ELA3B elisa kit

Disease Name	Pubmed Publications
Exocrine Pancreatic Insufficiency Antibodies	>1 publications with ELA3B and Exocrine Pancreatic Insufficiency

Organs/Tissues associated with ELA3B elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>5 publications with ELA3B and Pancreas