NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit for Human

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NKX6-1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKX6-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NKX6-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKX6-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NKX6-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of NKX6-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS453054 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NKX6-1. The ELISA analytical biochemical technique of the MBS453054 kit is based on NKX6-1 antibody-NKX6-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NKX6-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NKX6-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).