Lymphocyte Antigen 9 (LY9) ELISA Kit for Human

Name: Human LY9 ELISA Kit; Lymphocyte Antigen 9; MyBioSource
Brand: MyBioSource
SKU: MBS453153
Price: 445 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS453153

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LY9 in human tissue homogenates, cell lysates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated… More details

$445 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Lymphocyte Antigen 9 (LY9), ELISA Kit

Full Product Name

Human Lymphocyte Antigen 9 (LY9) ELISA Kit

Product Gene Name

LY9 elisa kit

Product Synonym Names

RASI-1; MMP18; Occasionally Referred To As Stromelysin-4

Species Reactivity

Human

Assay Type

Sandwich

Samples

Tissue homogenates, cell lysates and other biological fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.128ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of LY9. No significant cross-reactivity or interference between LY9 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LY9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LY9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

71,961 Da

OMIM

AF244129 mRNA

3D Structure

ModBase 3D Structure for Q9HBG7

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of LY9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LY9 purchase

MBS453153 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lymphocyte Antigen 9 (LY9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LY9. The ELISA analytical biochemical technique of the MBS453153 kit is based on LY9 antibody-LY9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LY9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LY9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LY9. It may not necessarily be applicable to this product.

NCBI GI #

75991709

NCBI GeneID

4063

NCBI Accession #

NP_001028839.1

UniProt Primary Accession #

Q9HBG7

UniProt Secondary Accession #

Q14775; Q5VYI3; Q6P2J4; Q9H4N5; Q9NQ24; A8K7N3

UniProt Related Accession #

Q9HBG7

GenBank Nucleotide #

NM_001033667.2

NCBI Official Full Name

T-lymphocyte surface antigen Ly-9 isoform b

NCBI Official Synonym Full Names

lymphocyte antigen 9

NCBI Official Symbol

LY9

NCBI Official Synonym Symbols

hly9; mLY9; CD229; SLAMF3

UniProt Protein Name

T-lymphocyte surface antigen Ly-9

UniProt Synonym Protein Names

Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD_antigen: CD229

Protein Family

T-lymphocyte surface antigen

UniProt Gene Name

LY9

UniProt Synonym Gene Names

SLAMF3

NCBI Protein Information

T-lymphocyte surface antigen Ly-9

NCBI Summary for LY9

LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008]

UniProt Comments for LY9

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed:22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed:22989874). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4+ T-cells. May negatively regulate the size of thymic innate CD8+ T-cells and the development of invariant natural killer T (iNKT) cells ().

Research Articles on LY9

1. the results presented here suggest that the tumor suppressor potential of SLAMF3 occurs through activation of Retinoblastoma protein that represses PLK1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LY9 elisa kit

Reference Product	PubMed Publications
SH2D1A elisa kit	>16 publications with LY9 and SH2D1A
CD48 elisa kit	>15 publications with LY9 and CD48
CD2 elisa kit	>13 publications with LY9 and CD2
FYN elisa kit	>3 publications with LY9 and FYN
SH2D1B elisa kit	>2 publications with LY9 and SH2D1B
GRB2 elisa kit	>1 publications with LY9 and GRB2

Diseases associated with LY9 elisa kit

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>26 publications with LY9 and Immune System Diseases
Vascular Diseases Antibodies	>5 publications with LY9 and Vascular Diseases
Cardiovascular Diseases Antibodies	>4 publications with LY9 and Cardiovascular Diseases
Carcinoma Antibodies	>3 publications with LY9 and Carcinoma
Kidney Diseases Antibodies	>1 publications with LY9 and Kidney Diseases
Neoplasms, Experimental Antibodies	>1 publications with LY9 and Neoplasms, Experimental
Cell Transformation, Neoplastic Antibodies	>1 publications with LY9 and Cell Transformation, Neoplastic
Necrosis Antibodies	>1 publications with LY9 and Necrosis

Organs/Tissues associated with LY9 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>43 publications with LY9 and Blood
Bone Marrow Antibodies	>11 publications with LY9 and Bone Marrow
Spleen Antibodies	>4 publications with LY9 and Spleen
Thymus Antibodies	>3 publications with LY9 and Thymus
Lymph Node Antibodies	>2 publications with LY9 and Lymph Node
Pharynx Antibodies	>1 publications with LY9 and Pharynx
Connective Tissue Antibodies	>1 publications with LY9 and Connective Tissue