Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit for Human

Name: Human GPX3 ELISA Kit; Glutathione Peroxidase 3, Plasma; MyBioSource
Brand: MyBioSource
SKU: MBS453731
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS453731

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPX3 in human serum, plasma and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glutathione Peroxidase 3, Plasma (GPX3), ELISA Kit

Full Product Name

Human Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit

Product Gene Name

GPX3 elisa kit

Product Synonym Names

GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma and other biological fluids.

Detection Range

6.25-400ng/mL

Sensitivity

2.42ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of GPX3. No significant cross-reactivity or interference between GPX3 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GPX3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GPX3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

25,552 Da

OMIM

D00632 mRNA

3D Structure

ModBase 3D Structure for P22352

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of GPX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPX3 purchase

MBS453731 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPX3. The ELISA analytical biochemical technique of the MBS453731 kit is based on GPX3 antibody-GPX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPX3. It may not necessarily be applicable to this product.

NCBI GI #

1048328959

NCBI GeneID

2878

NCBI Accession #

NP_001316719.1

UniProt Primary Accession #

P22352

UniProt Secondary Accession #

O43787; Q86W78; Q9NZ74; Q9UEL1

UniProt Related Accession #

P22352

GenBank Nucleotide #

NM_001329790.1

NCBI Official Full Name

glutathione peroxidase 3 isoform 2

NCBI Official Synonym Full Names

glutathione peroxidase 3

NCBI Official Symbol

GPX3

NCBI Official Synonym Symbols

GPx-P; GSHPx-3; GSHPx-P

UniProt Protein Name

Glutathione peroxidase 3

UniProt Synonym Protein Names

Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P

Protein Family

Probable glutathione peroxidase

UniProt Gene Name

GPX3

UniProt Synonym Gene Names

GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P

NCBI Protein Information

glutathione peroxidase 3

NCBI Summary for GPX3

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

UniProt Comments for GPX3

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Research Articles on GPX3

1. GPX1*Pro198Leu AND GPX3 rs2070593 as genetic risk markers for Italian asthmatic patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPX3 elisa kit

Reference Product	PubMed Publications
TNF elisa kit	>12 publications with GPX3 and TNF
SOD3 elisa kit	>11 publications with GPX3 and SOD3
GSTP1 elisa kit	>10 publications with GPX3 and GSTP1
GSTM3 elisa kit	>4 publications with GPX3 and GSTM3
GSTO1 elisa kit	>3 publications with GPX3 and GSTO1
GSTO2 elisa kit	>1 publications with GPX3 and GSTO2
GSTT1 elisa kit	>1 publications with GPX3 and GSTT1

Pathways associated with GPX3 elisa kit

Products by Pathway	Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
Detoxification Of Reactive Oxygen Species Pathway antibodies	Detoxification Of Reactive Oxygen Species Pathway Diagram
Folate Metabolism Pathway antibodies	Folate Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Metapathway Biotransformation antibodies	Metapathway Biotransformation Diagram
NRF2 Pathway antibodies	NRF2 Pathway Diagram

Diseases associated with GPX3 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>36 publications with GPX3 and Cardiovascular Diseases
Inflammation Antibodies	>29 publications with GPX3 and Inflammation
Adenocarcinoma Antibodies	>25 publications with GPX3 and Adenocarcinoma
Disease Models, Animal Antibodies	>23 publications with GPX3 and Disease Models, Animal
Necrosis Antibodies	>20 publications with GPX3 and Necrosis
Prostatic Neoplasms Antibodies	>15 publications with GPX3 and Prostatic Neoplasms
Obesity Antibodies	>13 publications with GPX3 and Obesity
Liver Diseases Antibodies	>13 publications with GPX3 and Liver Diseases
Lung Diseases Antibodies	>12 publications with GPX3 and Lung Diseases
Insulin Resistance Antibodies	>11 publications with GPX3 and Insulin Resistance

Organs/Tissues associated with GPX3 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>128 publications with GPX3 and Blood
Liver Antibodies	>58 publications with GPX3 and Liver
Kidney Antibodies	>38 publications with GPX3 and Kidney
Muscle Antibodies	>32 publications with GPX3 and Muscle
Lung Antibodies	>27 publications with GPX3 and Lung
Prostate Antibodies	>21 publications with GPX3 and Prostate
Thyroid Antibodies	>19 publications with GPX3 and Thyroid
Heart Antibodies	>19 publications with GPX3 and Heart
Brain Antibodies	>16 publications with GPX3 and Brain
Adipose Tissue Antibodies	>13 publications with GPX3 and Adipose Tissue