Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit for Human

Name: Human HIF2a ELISA Kit; Hypoxia Inducible Factor 2 Alpha; MyBioSource
Brand: MyBioSource
SKU: MBS454014
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS454014

MBS454014 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit target analytes in biological samples. The concentration gradients of… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Hypoxia Inducible Factor 2 Alpha (HIF2a), ELISA Kit

Full Product Name

Human Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

Product Gene Name

HIF2a elisa kit

Product Synonym Names

EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.053ng/mL

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Molecular Weight

96,459 Da

OMIM

U81984 mRNA

3D Structure

ModBase 3D Structure for Q99814

Intra Assay

CV<10%

Inter Assay

CV<12%

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of HIF2a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIF2a. It may not necessarily be applicable to this product.

NCBI GI #

40254439

NCBI GeneID

2034

NCBI Accession #

NP_001421.2

UniProt Primary Accession #

Q99814

UniProt Secondary Accession #

Q86VA2; Q99630

UniProt Related Accession #

Q99814

GenBank Nucleotide #

NM_001430.4

NCBI Official Full Name

endothelial PAS domain-containing protein 1

NCBI Official Synonym Full Names

endothelial PAS domain protein 1

NCBI Official Symbol

EPAS1

NCBI Official Synonym Symbols

HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

UniProt Protein Name

Endothelial PAS domain-containing protein 1

UniProt Synonym Protein Names

Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2

UniProt Gene Name

EPAS1

UniProt Synonym Gene Names

BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha

NCBI Protein Information

endothelial PAS domain-containing protein 1

NCBI Summary for HIF2a

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

UniProt Comments for HIF2a

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Research Articles on HIF2a

1. Abnormal upregulation of HIF-2alpha served as a key switch to direct TSPCs differentiation into osteochondral-lineage rather than teno-lineage. Notably, Scleraxis (Scx), an essential tendon specific transcription factor, was suppressed on constitutive activation of HIF-2alpha and mediated the effect of HIF-2alpha on TSPCs fate decision.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HIF2a elisa kit

Reference Product	PubMed Publications
VHL elisa kit	>32 publications with HIF2a and VHL
EGLN1 elisa kit	>30 publications with HIF2a and EGLN1
ARNT elisa kit	>14 publications with HIF2a and ARNT
EPO elisa kit	>8 publications with HIF2a and EPO
FLT1 elisa kit	>5 publications with HIF2a and FLT1
EGLN2 elisa kit	>2 publications with HIF2a and EGLN2
ARNT2 elisa kit	>2 publications with HIF2a and ARNT2
EGLN3 elisa kit	>1 publications with HIF2a and EGLN3

Pathways associated with HIF2a elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Amplification And Expansion Of Oncogenic Pathways As Metastatic Traits antibodies	Amplification And Expansion Of Oncogenic Pathways As Metastatic Traits Diagram
Cellular Response To Hypoxia Pathway antibodies	Cellular Response To Hypoxia Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
HIF-2-alpha Transcription Factor Network Pathway antibodies	HIF-2-alpha Transcription Factor Network Pathway Diagram
Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies	Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies	Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
PTK6 Expression Pathway antibodies	PTK6 Expression Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram

Diseases associated with HIF2a elisa kit

Disease Name	Pubmed Publications
Carcinoma, Renal Cell Antibodies	>16 publications with HIF2a and Carcinoma, Renal Cell
Disease Models, Animal Antibodies	>14 publications with HIF2a and Disease Models, Animal
Lung Diseases Antibodies	>11 publications with HIF2a and Lung Diseases
Kidney Diseases Antibodies	>10 publications with HIF2a and Kidney Diseases
Inflammation Antibodies	>9 publications with HIF2a and Inflammation
Necrosis Antibodies	>7 publications with HIF2a and Necrosis
Hypertension Antibodies	>7 publications with HIF2a and Hypertension
Lung Neoplasms Antibodies	>6 publications with HIF2a and Lung Neoplasms
Hypertrophy Antibodies	>5 publications with HIF2a and Hypertrophy
Neoplasms, Experimental Antibodies	>5 publications with HIF2a and Neoplasms, Experimental

Organs/Tissues associated with HIF2a elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>48 publications with HIF2a and Vascular
Blood Antibodies	>47 publications with HIF2a and Blood
Lung Antibodies	>20 publications with HIF2a and Lung
Kidney Antibodies	>20 publications with HIF2a and Kidney
Heart Antibodies	>18 publications with HIF2a and Heart
Liver Antibodies	>16 publications with HIF2a and Liver
Muscle Antibodies	>11 publications with HIF2a and Muscle
Embryonic Tissue Antibodies	>11 publications with HIF2a and Embryonic Tissue
Adrenal Gland Antibodies	>11 publications with HIF2a and Adrenal Gland
Brain Antibodies	>11 publications with HIF2a and Brain