Kisspeptin 1 (KISS1) ELISA Kit for Human

Name: Human KISS1 ELISA Kit; Kisspeptin 1; MyBioSource
Brand: MyBioSource
SKU: MBS454153
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS454153

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KISS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Kisspeptin 1 (KISS1), ELISA Kit

Full Product Name

Human Kisspeptin 1 (KISS1) ELISA Kit

Product Gene Name

KISS1 elisa kit

Product Synonym Names

Metastin; KiSS-1 Metastasis-Suppressor

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

< 12.9pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of KISS1. No significant cross-reactivity or interference between KISS1 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KISS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KISS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

14,705 Da

OMIM

U43527 mRNA

3D Structure

ModBase 3D Structure for Q15726

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of KISS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KISS1 purchase

MBS454153 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kisspeptin 1 (KISS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KISS1. The ELISA analytical biochemical technique of the MBS454153 kit is based on KISS1 antibody-KISS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KISS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KISS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KISS1. It may not necessarily be applicable to this product.

NCBI GI #

116829964

NCBI GeneID

3814

NCBI Accession #

NP_002247.3

UniProt Primary Accession #

Q15726

UniProt Secondary Accession #

Q9HBP1; A8K6N0

UniProt Related Accession #

Q15726

GenBank Nucleotide #

NM_002256.3

NCBI Official Full Name

metastasis-suppressor KiSS-1 preproprotein

NCBI Official Synonym Full Names

KiSS-1 metastasis-suppressor

NCBI Official Symbol

KISS1

NCBI Official Synonym Symbols

HH13; KiSS-1

UniProt Protein Name

Metastasis-suppressor KiSS-1

UniProt Synonym Protein Names

Kisspeptin-1

Protein Family

Metastasis-suppressor

UniProt Gene Name

KISS1

NCBI Protein Information

metastasis-suppressor KiSS-1

NCBI Summary for KISS1

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012]

UniProt Comments for KISS1

Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca2+ levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.

Research Articles on KISS1

1. There is an increasing appreciation that kisspeptin may act as a signal transmitter between metabolic status and reproductive function

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KISS1 elisa kit

Reference Product	PubMed Publications
KISS1R elisa kit	>421 publications with KISS1 and KISS1R
GNRH1 elisa kit	>49 publications with KISS1 and GNRH1
TAC3 elisa kit	>25 publications with KISS1 and TAC3
GNRHR elisa kit	>24 publications with KISS1 and GNRHR
TACR3 elisa kit	>22 publications with KISS1 and TACR3
GNRH2 elisa kit	>18 publications with KISS1 and GNRH2
PROKR2 elisa kit	>9 publications with KISS1 and PROKR2
NPFFR1 elisa kit	>1 publications with KISS1 and NPFFR1

Pathways associated with KISS1 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with KISS1 elisa kit

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>109 publications with KISS1 and Neoplasm Metastasis
Genital Diseases, Female Antibodies	>68 publications with KISS1 and Genital Diseases, Female
Male Urogenital Diseases Antibodies	>67 publications with KISS1 and Male Urogenital Diseases
Puberty, Precocious Antibodies	>64 publications with KISS1 and Puberty, Precocious
Infertility, Female Antibodies	>62 publications with KISS1 and Infertility, Female
Breast Neoplasms Antibodies	>50 publications with KISS1 and Breast Neoplasms
Puberty, Delayed Antibodies	>47 publications with KISS1 and Puberty, Delayed
Infertility, Male Antibodies	>40 publications with KISS1 and Infertility, Male
Adenocarcinoma Antibodies	>35 publications with KISS1 and Adenocarcinoma
Disorders of Sex Development Antibodies	>25 publications with KISS1 and Disorders of Sex Development

Organs/Tissues associated with KISS1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>726 publications with KISS1 and Brain
Placenta Antibodies	>70 publications with KISS1 and Placenta
Testis Antibodies	>68 publications with KISS1 and Testis
Embryonic Tissue Antibodies	>28 publications with KISS1 and Embryonic Tissue
Prostate Antibodies	>24 publications with KISS1 and Prostate
Lung Antibodies	>17 publications with KISS1 and Lung
Bone Antibodies	>15 publications with KISS1 and Bone
Skin Antibodies	>12 publications with KISS1 and Skin
Heart Antibodies	>11 publications with KISS1 and Heart
Intestine Antibodies	>7 publications with KISS1 and Intestine