Creatine Kinase, Mitochondrial 1a (CKMT1A) ELISA Kit for Human

Name: Human CKMT1A ELISA Kit; Creatine Kinase, Mitochondrial 1a; MyBioSource
Brand: MyBioSource
SKU: MBS454456
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS454456

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CKMT1A in human serum, plasma, tissue homogenates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been… More details

$480 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Creatine Kinase, Mitochondrial 1A (CKMT1A), ELISA Kit

Full Product Name

Human Creatine Kinase, Mitochondrial 1A (CKMT1A) ELISA Kit

Product Gene Name

CKMT1A elisa kit

Product Synonym Names

CKMT CKMT1-A Mia-CK U-MtCK Creatine kinase U-type, mitochondrial Ubiquitous mitochondrial creatine kinase Acidic-type mitochondrial creatine kinase

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids.

Detection Range

1.56-100ng/mL

Sensitivity

0.57ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of CKMT1A. No significant cross-reactivity or interference between CKMT1A and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CKMT1A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CKMT1A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

50,421 Da

OMIM

J04469 Genomic DNA

3D Structure

ModBase 3D Structure for P12532

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of CKMT1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CKMT1A purchase

MBS454456 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine Kinase, Mitochondrial 1A (CKMT1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CKMT1A. The ELISA analytical biochemical technique of the MBS454456 kit is based on CKMT1A antibody-CKMT1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CKMT1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CKMT1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CKMT1A. It may not necessarily be applicable to this product.

NCBI GI #

66346730

NCBI GeneID

548596

NCBI Accession #

NP_001015001.1

UniProt Primary Accession #

P12532

UniProt Secondary Accession #

Q0VAM3; Q32NF6; Q53FC4; B4DIT8; B7ZA09

UniProt Related Accession #

P12532

GenBank Nucleotide #

NM_001015001.2

NCBI Official Full Name

creatine kinase U-type, mitochondrial isoform 1

NCBI Official Synonym Full Names

creatine kinase, mitochondrial 1A

NCBI Official Symbol

CKMT1A

NCBI Official Synonym Symbols

CKMT1; U-MtCK; mia-CK

UniProt Protein Name

Creatine kinase U-type, mitochondrial

UniProt Synonym Protein Names

Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK

UniProt Gene Name

CKMT1A

UniProt Synonym Gene Names

CKMT; Mia-CK; U-MtCK

NCBI Protein Information

creatine kinase U-type, mitochondrial

NCBI Summary for CKMT1A

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]

UniProt Comments for CKMT1A

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Research Articles on CKMT1A

1. CKMT1 is a key regulator of the permeability transition pore through a complex that is distinct from the classical permeability transition pore.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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STRC elisa kit	>1 publications with CKMT1A and STRC

Pathways associated with CKMT1A elisa kit

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Diseases associated with CKMT1A elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1 publications with CKMT1A and Nervous System Diseases
Chromosome Aberrations Antibodies	>1 publications with CKMT1A and Chromosome Aberrations

Organs/Tissues associated with CKMT1A elisa kit

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>1 publications with CKMT1A and Nerve
Connective Tissue Antibodies	>1 publications with CKMT1A and Connective Tissue