Nephrin (NPHN) ELISA Kit for Human

Name: Human NPHN ELISA Kit; Nephrin; MyBioSource
Brand: MyBioSource
SKU: MBS454926
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS454926

MBS454926 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nephrin (NPHN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or… More details

$480 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Nephrin (NPHN), ELISA Kit

Full Product Name

Human Nephrin (NPHN) ELISA Kit

Product Gene Name

NPHN elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.123ng/mL

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Molecular Weight

130,546 Da

OMIM

AF035835 mRNA

3D Structure

ModBase 3D Structure for O60500

Intra Assay

CV<10%

Inter Assay

CV<12%

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of NPHN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NPHN. It may not necessarily be applicable to this product.

NCBI GI #

4758822

NCBI GeneID

4868

NCBI Accession #

NP_004637.1

UniProt Primary Accession #

O60500

UniProt Secondary Accession #

A6NDH2; C3RX61

UniProt Related Accession #

O60500

GenBank Nucleotide #

NM_004646.3

NCBI Official Full Name

nephrin

NCBI Official Synonym Full Names

NPHS1, nephrin

NCBI Official Symbol

NPHS1

NCBI Official Synonym Symbols

CNF; NPHN; nephrin

UniProt Protein Name

Nephrin

UniProt Synonym Protein Names

Renal glomerulus-specific cell adhesion receptor

UniProt Gene Name

NPHS1

UniProt Synonym Gene Names

NPHN

NCBI Protein Information

nephrin

NCBI Summary for NPHN

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

UniProt Comments for NPHN

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion ().

Research Articles on NPHN

1. The classical form is CNF, which is caused by mutations in the nephrin gene (NPHS1), leading to massive proteinuria, hypoproteinemia and edema in the newborn period

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NPHN elisa kit

Reference Product	PubMed Publications
NPHS2 elisa kit	>92 publications with NPHN and NPHS2
WT1 elisa kit	>54 publications with NPHN and WT1
CD2AP elisa kit	>26 publications with NPHN and CD2AP
TRPC6 elisa kit	>12 publications with NPHN and TRPC6
TJP1 elisa kit	>6 publications with NPHN and TJP1
SRC elisa kit	>4 publications with NPHN and SRC
KIRREL elisa kit	>4 publications with NPHN and KIRREL
FYN elisa kit	>1 publications with NPHN and FYN

Pathways associated with NPHN elisa kit

Products by Pathway	Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Nephrin Interactions Pathway antibodies	Nephrin Interactions Pathway Diagram
Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies	Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
Primary Focal Segmental Glomerulosclerosis FSGS Pathway antibodies	Primary Focal Segmental Glomerulosclerosis FSGS Pathway Diagram

Diseases associated with NPHN elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>170 publications with NPHN and Kidney Diseases
Nephrosis Antibodies	>131 publications with NPHN and Nephrosis
Proteinuria Antibodies	>87 publications with NPHN and Proteinuria
Glomerulosclerosis, Focal Segmental Antibodies	>50 publications with NPHN and Glomerulosclerosis, Focal Segmental
Glomerulonephritis Antibodies	>45 publications with NPHN and Glomerulonephritis
Nephritis Antibodies	>44 publications with NPHN and Nephritis
Renal Insufficiency Antibodies	>19 publications with NPHN and Renal Insufficiency
Kidney Failure, Chronic Antibodies	>18 publications with NPHN and Kidney Failure, Chronic
Nephrosis, congenital Antibodies	>16 publications with NPHN and Nephrosis, congenital
Disease Models, Animal Antibodies	>13 publications with NPHN and Disease Models, Animal

Organs/Tissues associated with NPHN elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>152 publications with NPHN and Kidney
Pancreas Antibodies	>8 publications with NPHN and Pancreas
Brain Antibodies	>6 publications with NPHN and Brain
Thymus Antibodies	>3 publications with NPHN and Thymus
Testis Antibodies	>2 publications with NPHN and Testis