Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit for Human

Name: Human PCK1 ELISA Kit; Phosphoenolpyruvate Carboxykinase 1, Soluble; MyBioSource
Brand: MyBioSource
SKU: MBS455356
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS455356

MBS455356 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit target analytes in biological samples. The concentration… More details

$480 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), ELISA Kit

Full Product Name

Human Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit

Product Gene Name

PCK1 elisa kit

Product Synonym Names

PEPCK1; PEPCKC; Cytosolic Phosphoenolpyruvate Carboxykinase; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.051ng/mL

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Molecular Weight

34,156 Da

OMIM

L05144 mRNA

3D Structure

ModBase 3D Structure for P35558

Intra Assay

CV<10%

Inter Assay

CV<12%

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PCK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PCK1. It may not necessarily be applicable to this product.

NCBI GI #

187281517

NCBI GeneID

5105

NCBI Accession #

NP_002582.3

UniProt Primary Accession #

P35558

UniProt Secondary Accession #

Q8TCA3; Q9UJD2; A8K437; B4DT64

UniProt Related Accession #

P35558

GenBank Nucleotide #

NM_002591.3

NCBI Official Full Name

phosphoenolpyruvate carboxykinase, cytosolic

NCBI Official Synonym Full Names

phosphoenolpyruvate carboxykinase 1

NCBI Official Symbol

PCK1

NCBI Official Synonym Symbols

PEPCK1; PEPCKC; PEPCK-C

UniProt Protein Name

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Protein Family

Phosphoenolpyruvate carboxykinase

UniProt Gene Name

PCK1

UniProt Synonym Gene Names

PEPCK-C

NCBI Protein Information

phosphoenolpyruvate carboxykinase, cytosolic [GTP]

NCBI Summary for PCK1

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for PCK1

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Research Articles on PCK1

1. an extended binding site in the catalytic cleft of cPEPCK which is used by 3-MPA to inhibit cPEPCK non-competitively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PCK1 elisa kit

Reference Product	PubMed Publications
PPARGC1A elisa kit	>17 publications with PCK1 and PPARGC1A
FOXO1 elisa kit	>15 publications with PCK1 and FOXO1
PPARG elisa kit	>10 publications with PCK1 and PPARG
FABP4 elisa kit	>2 publications with PCK1 and FABP4
MDH1 elisa kit	>1 publications with PCK1 and MDH1

Pathways associated with PCK1 elisa kit

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
Abacavir Metabolism Pathway antibodies	Abacavir Metabolism Pathway Diagram
Abacavir Transport And Metabolism Pathway antibodies	Abacavir Transport And Metabolism Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Angiopoietin Like Protein 8 Regulatory Pathway antibodies	Angiopoietin Like Protein 8 Regulatory Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram

Diseases associated with PCK1 elisa kit

Disease Name	Pubmed Publications
Insulin Resistance Antibodies	>40 publications with PCK1 and Insulin Resistance
Fatty Liver Antibodies	>38 publications with PCK1 and Fatty Liver
Liver Diseases Antibodies	>31 publications with PCK1 and Liver Diseases
Obesity Antibodies	>26 publications with PCK1 and Obesity
Hyperglycemia Antibodies	>21 publications with PCK1 and Hyperglycemia
Inflammation Antibodies	>18 publications with PCK1 and Inflammation
Disease Models, Animal Antibodies	>15 publications with PCK1 and Disease Models, Animal
Carcinoma, Hepatocellular Antibodies	>9 publications with PCK1 and Carcinoma, Hepatocellular
Weight Gain Antibodies	>8 publications with PCK1 and Weight Gain
Glucose Intolerance Antibodies	>8 publications with PCK1 and Glucose Intolerance

Organs/Tissues associated with PCK1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>114 publications with PCK1 and Liver
Blood Antibodies	>67 publications with PCK1 and Blood
Adipose Tissue Antibodies	>36 publications with PCK1 and Adipose Tissue
Muscle Antibodies	>19 publications with PCK1 and Muscle
Kidney Antibodies	>13 publications with PCK1 and Kidney
Brain Antibodies	>9 publications with PCK1 and Brain
Pancreas Antibodies	>8 publications with PCK1 and Pancreas
Intestine Antibodies	>8 publications with PCK1 and Intestine
Ovary Antibodies	>6 publications with PCK1 and Ovary
Heart Antibodies	>4 publications with PCK1 and Heart