Cytochrome P450 2b6 (CYP2B6) ELISA Kit for Human

Name: Human CYP2B6 ELISA Kit; Cytochrome P450 2b6; MyBioSource
Brand: MyBioSource
SKU: MBS456049
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS456049

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP2B6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Cytochrome P450 2B6 (CYP2B6), ELISA Kit

Full Product Name

Human Cytochrome P450 2B6 (CYP2B6) ELISA Kit

Product Gene Name

CYP2B6 elisa kit

Product Synonym Names

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human tissue homogenates, cell lysates or other biological fluids

Detection Range

0.781-50ng/mL

Sensitivity

< 0.31ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of CYP2B6. No significant cross-reactivity or interference between CYP2B6 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP2B6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP2B6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

33,385 Da

OMIM

M29874 mRNA

3D Structure

ModBase 3D Structure for P20813

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of CYP2B6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP2B6 purchase

MBS456049 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 2B6 (CYP2B6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP2B6. The ELISA analytical biochemical technique of the MBS456049 kit is based on CYP2B6 antibody-CYP2B6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP2B6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP2B6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CYP2B6. It may not necessarily be applicable to this product.

NCBI GI #

7949031

NCBI GeneID

1555

NCBI Accession #

NP_000758.1

UniProt Primary Accession #

P20813

UniProt Secondary Accession #

Q2V565; Q9UK46; B4DWP3

UniProt Related Accession #

P20813

GenBank Nucleotide #

NM_000767.4

NCBI Official Full Name

cytochrome P450 2B6

NCBI Official Synonym Full Names

cytochrome P450 family 2 subfamily B member 6

NCBI Official Symbol

CYP2B6

NCBI Official Synonym Symbols

CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6

UniProt Protein Name

Cytochrome P450 2B6

UniProt Synonym Protein Names

1,4-cineole 2-exo-monooxygenase; CYPIIB6; Cytochrome P450 IIB1

Protein Family

Cytochrome

UniProt Gene Name

CYP2B6

NCBI Protein Information

cytochrome P450 2B6

NCBI Summary for CYP2B6

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

UniProt Comments for CYP2B6

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.

Research Articles on CYP2B6

1. CYP2B6*6 and *9 variant alleles had a significant decreasing effect on sertraline metabolism in major depression patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYP2B6 elisa kit

Reference Product	PubMed Publications
UGT1A1 elisa kit	>54 publications with CYP2B6 and UGT1A1
UGT1A9 elisa kit	>23 publications with CYP2B6 and UGT1A9
NR1I2 elisa kit	>19 publications with CYP2B6 and NR1I2
UGT1A6 elisa kit	>9 publications with CYP2B6 and UGT1A6
UGT1A4 elisa kit	>8 publications with CYP2B6 and UGT1A4
UGT1A3 elisa kit	>7 publications with CYP2B6 and UGT1A3
UGT1A7 elisa kit	>2 publications with CYP2B6 and UGT1A7

Pathways associated with CYP2B6 elisa kit

Products by Pathway	Pathway Diagram
Angiopoietin Like Protein 8 Regulatory Pathway antibodies	Angiopoietin Like Protein 8 Regulatory Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
CYP2E1 Reactions Pathway antibodies	CYP2E1 Reactions Pathway Diagram
Constitutive Androstane Receptor Pathway antibodies	Constitutive Androstane Receptor Pathway Diagram
Cytochrome P450 - Arranged By Substrate Type Pathway antibodies	Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
Diclofenac Metabolic Pathway antibodies	Diclofenac Metabolic Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram

Diseases associated with CYP2B6 elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>91 publications with CYP2B6 and Liver Diseases
Nervous System Diseases Antibodies	>48 publications with CYP2B6 and Nervous System Diseases
Liver Neoplasms Antibodies	>45 publications with CYP2B6 and Liver Neoplasms
Poisoning Antibodies	>40 publications with CYP2B6 and Poisoning
Drug-Related Side Effects and Adverse Reactions Antibodies	>38 publications with CYP2B6 and Drug-Related Side Effects and Adverse Reactions
Pain Antibodies	>29 publications with CYP2B6 and Pain
Carcinoma, Hepatocellular Antibodies	>27 publications with CYP2B6 and Carcinoma, Hepatocellular
Drug-Induced Liver Injury Antibodies	>26 publications with CYP2B6 and Drug-Induced Liver Injury
Hepatitis Antibodies	>20 publications with CYP2B6 and Hepatitis
Kidney Diseases Antibodies	>17 publications with CYP2B6 and Kidney Diseases

Organs/Tissues associated with CYP2B6 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>795 publications with CYP2B6 and Liver
Kidney Antibodies	>43 publications with CYP2B6 and Kidney
Intestine Antibodies	>32 publications with CYP2B6 and Intestine
Muscle Antibodies	>5 publications with CYP2B6 and Muscle
Mammary Gland Antibodies	>1 publications with CYP2B6 and Mammary Gland