Neuraminidase (NEU) ELISA Kit for Human

Name: Human NEU ELISA Kit; Neuraminidase; MyBioSource
Brand: MyBioSource
SKU: MBS456114
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS456114

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEU. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Neuraminidase (NEU), ELISA Kit

Full Product Name

Human Neuraminidase (NEU) ELISA Kit

Product Gene Name

NEU elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids

Detection Range

0.312-20ng/mL

Sensitivity

< 0.126ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of NEU. No significant cross-reactivity or interference between NEU and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEU were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEU were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

45,467 Da

OMIM

AF040958 mRNA

3D Structure

ModBase 3D Structure for Q99519

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of NEU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEU purchase

MBS456114 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neuraminidase (NEU) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEU. The ELISA analytical biochemical technique of the MBS456114 kit is based on NEU antibody-NEU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NEU. It may not necessarily be applicable to this product.

NCBI GI #

4557791

NCBI GeneID

4758

NCBI Accession #

NP_000425.1

UniProt Primary Accession #

Q99519

UniProt Related Accession #

Q99519

GenBank Nucleotide #

NM_000434.3

NCBI Official Full Name

sialidase-1

NCBI Official Synonym Full Names

neuraminidase 1

NCBI Official Symbol

NEU1

NCBI Official Synonym Symbols

NEU; NANH; SIAL1

UniProt Protein Name

Sialidase-1

UniProt Synonym Protein Names

Acetylneuraminyl hydrolase; G9 sialidase; Lysosomal sialidase; N-acetyl-alpha-neuraminidase 1

UniProt Gene Name

NEU1

UniProt Synonym Gene Names

NANH

NCBI Protein Information

sialidase-1

NCBI Summary for NEU

The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]

UniProt Comments for NEU

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.

Research Articles on NEU

1. NEU1 siRNA can effectively inhibit proliferation, apoptosis, and invasion of human ovarian cancer cells by targeting lysosome and oxidative phosphorylation signaling, which can serve as a new target ovarian cancer treatment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NEU elisa kit

Reference Product	PubMed Publications
NEU3 elisa kit	>44 publications with NEU and NEU3
GLB1 elisa kit	>13 publications with NEU and GLB1
CTSA elisa kit	>10 publications with NEU and CTSA
EGFR elisa kit	>3 publications with NEU and EGFR

Pathways associated with NEU elisa kit

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies	Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
Glycosphingolipid Metabolism Pathway antibodies	Glycosphingolipid Metabolism Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Diseases associated with NEU elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>12 publications with NEU and Inflammation
Immune System Diseases Antibodies	>10 publications with NEU and Immune System Diseases
Liver Diseases Antibodies	>6 publications with NEU and Liver Diseases
Neuraminidase 1 deficiency Antibodies	>6 publications with NEU and Neuraminidase 1 deficiency
Kidney Diseases Antibodies	>5 publications with NEU and Kidney Diseases
Cardiovascular Diseases Antibodies	>5 publications with NEU and Cardiovascular Diseases
Seizures Antibodies	>5 publications with NEU and Seizures
Necrosis Antibodies	>3 publications with NEU and Necrosis
Edema Antibodies	>3 publications with NEU and Edema
Hypertension Antibodies	>2 publications with NEU and Hypertension

Organs/Tissues associated with NEU elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>32 publications with NEU and Blood
Brain Antibodies	>24 publications with NEU and Brain
Liver Antibodies	>11 publications with NEU and Liver
Lung Antibodies	>10 publications with NEU and Lung
Bone Antibodies	>10 publications with NEU and Bone
Muscle Antibodies	>9 publications with NEU and Muscle
Kidney Antibodies	>9 publications with NEU and Kidney
Nerve Antibodies	>8 publications with NEU and Nerve
Bone Marrow Antibodies	>8 publications with NEU and Bone Marrow
Skin Antibodies	>8 publications with NEU and Skin