Protease, Serine 1 (PRSS1) ELISA Kit for Human

Name: Human PRSS1 ELISA Kit; Protease, Serine 1; MyBioSource
Brand: MyBioSource
SKU: MBS456138
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS456138

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PRSS1 in human serum, plasma, tissue homogenates or other biological fluids. More details

$480 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Protease, Serine 1 (PRSS1), ELISA Kit

Full Product Name

Human Protease, Serine 1 (PRSS1) ELISA Kit

Product Gene Name

PRSS1 elisa kit

Species Reactivity

Human

Samples

human serum, plasma, tissue homogenates or other biological fluids

Detection Range

7.81500U/L

Sensitivity

<3.9U/L

Specificity

This assay has high sensitivity and excellent specificity for detection of PRSS1. No significant cross-reactivity or interference between PRSS1 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PRSS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PRSS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

26,558 Da

OMIM

M22612 mRNA

3D Structure

ModBase 3D Structure for P07477

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PRSS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRSS1 purchase

MBS456138 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protease, Serine 1 (PRSS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRSS1. The ELISA analytical biochemical technique of the MBS456138 kit is based on PRSS1 antibody-PRSS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRSS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRSS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PRSS1. It may not necessarily be applicable to this product.

NCBI GI #

4506145

NCBI GeneID

5644

NCBI Accession #

NP_002760.1

UniProt Primary Accession #

P07477

UniProt Secondary Accession #

Q5NV57; Q7M4N3; Q7M4N4; Q92955; Q9HAN4; Q9HAN5; Q9HAN6; Q9HAN7; A1A509; A6NJ71; B2R5I5

UniProt Related Accession #

P07477

GenBank Nucleotide #

NM_002769.4

NCBI Official Full Name

trypsin-1 preproprotein

NCBI Official Synonym Full Names

protease, serine 1

NCBI Official Symbol

PRSS1

NCBI Official Synonym Symbols

TRP1; TRY1; TRY4; TRYP1

UniProt Protein Name

Trypsin-1

UniProt Synonym Protein Names

Beta-trypsin; Cationic trypsinogen; Serine protease 1; Trypsin I

Protein Family

Anionic trypsin

UniProt Gene Name

PRSS1

UniProt Synonym Gene Names

TRP1; TRY1; TRYP1

NCBI Protein Information

trypsin-1

NCBI Summary for PRSS1

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]

UniProt Comments for PRSS1

Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.

Research Articles on PRSS1

1. Human anionic trypsinogen is controlled by CTRC in a manner that individual natural mutations are unlikely to increase stability enough to promote intra-pancreatic activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRSS1 elisa kit

Reference Product	PubMed Publications
SPINK1 elisa kit	>175 publications with PRSS1 and SPINK1
MMP1 elisa kit	>1 publications with PRSS1 and MMP1

Pathways associated with PRSS1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies	Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Vitamins And Cofactors Pathway antibodies	Metabolism Of Vitamins And Cofactors Pathway Diagram
Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies	Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with PRSS1 elisa kit

Disease Name	Pubmed Publications
Pancreatitis Antibodies	>266 publications with PRSS1 and Pancreatitis
Pancreatitis, Chronic Antibodies	>211 publications with PRSS1 and Pancreatitis, Chronic
Hereditary pancreatitis Antibodies	>113 publications with PRSS1 and Hereditary pancreatitis
Fibrosis Antibodies	>108 publications with PRSS1 and Fibrosis
Inflammation Antibodies	>27 publications with PRSS1 and Inflammation
Carcinoma Antibodies	>21 publications with PRSS1 and Carcinoma
Immune System Diseases Antibodies	>14 publications with PRSS1 and Immune System Diseases
Breast Neoplasms Antibodies	>6 publications with PRSS1 and Breast Neoplasms
Necrosis Antibodies	>6 publications with PRSS1 and Necrosis
Liver Diseases Antibodies	>5 publications with PRSS1 and Liver Diseases

Organs/Tissues associated with PRSS1 elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>105 publications with PRSS1 and Pancreas
Blood Antibodies	>46 publications with PRSS1 and Blood
Liver Antibodies	>13 publications with PRSS1 and Liver
Skin Antibodies	>4 publications with PRSS1 and Skin
Brain Antibodies	>4 publications with PRSS1 and Brain
Kidney Antibodies	>3 publications with PRSS1 and Kidney
Heart Antibodies	>3 publications with PRSS1 and Heart
Embryonic Tissue Antibodies	>2 publications with PRSS1 and Embryonic Tissue
Muscle Antibodies	>2 publications with PRSS1 and Muscle
Bone Antibodies	>2 publications with PRSS1 and Bone