• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Insulin Receptor Substrate 1 (IRS1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS456722

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IRS1 in human tissue homogenates, cell lysates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Insulin Receptor Substrate 1 (IRS1), ELISA Kit
Full Product Name
Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit
Product Gene Name
IRS1 elisa kit
Product Synonym Names
HIRS1
Species Reactivity
Human
Assay Type
Sandwich
Samples
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.147ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of IRS1. No significant cross-reactivity or interference between IRS1 and analogues was observed.
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IRS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IRS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
131,591 Da
OMIM
S85963 Genomic DNA
3D Structure
ModBase 3D Structure for P35568
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of IRS1 elisa kit

Human IRS1 ELISA Kit – Cat# MBS456722

Other Notes

Small volumes of IRS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRS1 purchase

MBS456722 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin Receptor Substrate 1 (IRS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IRS1. The ELISA analytical biochemical technique of the MBS456722 kit is based on IRS1 antibody-IRS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IRS1. It may not necessarily be applicable to this product.

NCBI GI #
5031805
NCBI GeneID
3667
NCBI Accession #
NP_005535.1
UniProt Primary Accession #
P35568
UniProt Related Accession #
P35568
GenBank Nucleotide #
NM_005544.2
NCBI Official Full Name
insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
UniProt Protein Name
Insulin receptor substrate 1
Protein Family
Isoleucine--tRNA ligase
UniProt Gene Name
IRS1
UniProt Synonym Gene Names
IRS-1
NCBI Protein Information
insulin receptor substrate 1

NCBI Summary for IRS1

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

UniProt Comments for IRS1

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: caveola; cytoplasm; cytosol; intracellular membrane-bound organelle; nucleus; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; insulin receptor binding; insulin-like growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoinositide 3-kinase binding; protein binding; protein kinase C binding; Ras guanyl-nucleotide exchange factor activity; SH2 domain binding; signal transducer activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: cellular response to insulin stimulus; glucose homeostasis; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; MAPKKK cascade; negative regulation of insulin receptor signaling pathway; negative regulation of insulin secretion; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of insulin receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; regulation of phosphoinositide 3-kinase cascade; response to insulin stimulus; response to peptide hormone stimulus; signal transduction

Disease: Diabetes Mellitus, Noninsulin-dependent
Show more

Research Articles on IRS1

1. The Arg(972) IRS-1 polymorphism is associated with increased risk and disease activity/severity of rheumatoid arthritis, and therefore may be a potential prognostic factor for RA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IRS1 elisa kit

 Reference Product  PubMed Publications
 IGF1R elisa kit  >268 publications with IRS1 and IGF1R
 GRB2 elisa kit  >140 publications with IRS1 and GRB2
 INSR elisa kit  >73 publications with IRS1 and INSR
 JAK2 elisa kit  >66 publications with IRS1 and JAK2
 PTPN11 elisa kit  >41 publications with IRS1 and PTPN11
 JAK1 elisa kit  >27 publications with IRS1 and JAK1
 MAPK8 elisa kit  >23 publications with IRS1 and MAPK8
 PIK3R1 elisa kit  >14 publications with IRS1 and PIK3R1
 PIK3CA elisa kit  >11 publications with IRS1 and PIK3CA
 SOCS1 elisa kit  >8 publications with IRS1 and SOCS1

Pathways associated with IRS1 elisa kit

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram

Diseases associated with IRS1 elisa kit

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >1445 publications with IRS1 and Insulin Resistance
 Hyperinsulinism Antibodies  >981 publications with IRS1 and Hyperinsulinism
 Diabetes Mellitus Antibodies  >579 publications with IRS1 and Diabetes Mellitus
 Obesity Antibodies  >490 publications with IRS1 and Obesity
 Diabetes Mellitus, Type 2 Antibodies  >400 publications with IRS1 and Diabetes Mellitus, Type 2
 Disease Models, Animal Antibodies  >237 publications with IRS1 and Disease Models, Animal
 Hyperglycemia Antibodies  >209 publications with IRS1 and Hyperglycemia
 Necrosis Antibodies  >206 publications with IRS1 and Necrosis
 Inflammation Antibodies  >177 publications with IRS1 and Inflammation
 Liver Diseases Antibodies  >158 publications with IRS1 and Liver Diseases

Organs/Tissues associated with IRS1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >949 publications with IRS1 and Muscle
 Liver Antibodies  >569 publications with IRS1 and Liver
 Connective Tissue Antibodies  >276 publications with IRS1 and Connective Tissue
 Pancreas Antibodies  >169 publications with IRS1 and Pancreas
 Heart Antibodies  >158 publications with IRS1 and Heart
 Brain Antibodies  >152 publications with IRS1 and Brain
 Ovary Antibodies  >125 publications with IRS1 and Ovary
 Kidney Antibodies  >112 publications with IRS1 and Kidney
 Bone Antibodies  >55 publications with IRS1 and Bone
 Nerve Antibodies  >50 publications with IRS1 and Nerve
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000