Interferon Alpha/Beta Receptor 2 (Ifna/Br2) (IFNa/bR2) ELISA Kit for Human

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IFNa/bR2 in human serum, plasma, urine and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNa/bR2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IFNa/bR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of IFNa/bR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS456874 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon Alpha/Beta Receptor 2 (IFNa/bR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFNa/bR2. The ELISA analytical biochemical technique of the MBS456874 kit is based on IFNa/bR2 antibody-IFNa/bR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNa/bR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNa/bR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).