C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit for Mouse

Name: Mouse ROS1 ELISA Kit; C-Ros Oncogene 1, Receptor Tyrosine Kinase; MyBioSource
Brand: MyBioSource
SKU: MBS456919
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS456919

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ROS1 in mouse serum, plasma, tissue homogenates or other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been… More details

$530 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), ELISA Kit

Full Product Name

Mouse C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit

Product Gene Name

ROS1 elisa kit

Product Synonym Names

MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates or other biological fluids

Detection Range

78.1-5000pg/mL

Sensitivity

<33pg/mL.

Specificity

This assay has high sensitivity and excellent specificity for detection of ROS1. No significant cross-reactivity or interference between ROS1 and analogues was observed.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ROS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ROS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

261,967 Da

3D Structure

ModBase 3D Structure for Q78DX7

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ROS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ROS1 purchase

MBS456919 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ROS1. The ELISA analytical biochemical technique of the MBS456919 kit is based on ROS1 antibody-ROS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ROS1. It may not necessarily be applicable to this product.

NCBI GI #

114326510

NCBI GeneID

19886

NCBI Accession #

NP_035412.2

UniProt Primary Accession #

Q78DX7

UniProt Secondary Accession #

Q60705

UniProt Related Accession #

Q78DX7

GenBank Nucleotide #

NM_011282.2

NCBI Official Full Name

proto-oncogene tyrosine-protein kinase ROS

NCBI Official Synonym Full Names

Ros1 proto-oncogene

NCBI Official Symbol

Ros1

NCBI Official Synonym Symbols

Ros-1; c-ros

UniProt Protein Name

Proto-oncogene tyrosine-protein kinase ROS

UniProt Synonym Protein Names

Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase

Protein Family

Protein

UniProt Gene Name

Ros1

UniProt Synonym Gene Names

Ros; Ros-1

NCBI Protein Information

proto-oncogene tyrosine-protein kinase ROS

UniProt Comments for ROS1

ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Sev family; TK group

Chromosomal Location of Human Ortholog: 10 B3|10 26.18 cM

Cellular Component: cell surface; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; kinase activity; nucleotide binding; protein kinase activity; protein phosphatase binding; protein tyrosine kinase activity; transferase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell differentiation; cell proliferation; columnar/cuboidal epithelial cell development; negative regulation of gene expression; peptidyl-tyrosine autophosphorylation; phosphorylation; protein amino acid phosphorylation; regulation of cell growth; regulation of ERK1 and ERK2 cascade; regulation of phosphate transport; regulation of TOR signaling; spermatogenesis

Research Articles on ROS1

1. GPX1-dependent alterations in oxido-reductive stress promote ROS1 activation and mediate vascular remodeling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ROS1 elisa kit

Reference Product	PubMed Publications
Ptpn6 elisa kit	>2 publications with ROS1 and Ptpn6
Cope elisa kit	>1 publications with ROS1 and Cope
Palld elisa kit	>1 publications with ROS1 and Palld

Diseases associated with ROS1 elisa kit

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>365 publications with ROS1 and Lung Neoplasms
Carcinoma, Non-Small-Cell Lung Antibodies	>232 publications with ROS1 and Carcinoma, Non-Small-Cell Lung
Adenocarcinoma of lung Antibodies	>72 publications with ROS1 and Adenocarcinoma of lung
Carcinoma, Squamous Cell Antibodies	>66 publications with ROS1 and Carcinoma, Squamous Cell
Glioma Antibodies	>22 publications with ROS1 and Glioma
Neoplasms, Experimental Antibodies	>22 publications with ROS1 and Neoplasms, Experimental
Melanoma Antibodies	>20 publications with ROS1 and Melanoma
Breast Neoplasms Antibodies	>17 publications with ROS1 and Breast Neoplasms
Liver Neoplasms Antibodies	>13 publications with ROS1 and Liver Neoplasms
Infertility, Male Antibodies	>12 publications with ROS1 and Infertility, Male

Organs/Tissues associated with ROS1 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>529 publications with ROS1 and Lung