Collagen Type III Alpha 1 (COL3a1) ELISA Kit for Mouse

Name: Mouse COL3a1 ELISA Kit; Collagen Type III Alpha 1; MyBioSource
Brand: MyBioSource
SKU: MBS457004
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS457004

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL3a1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$530 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Collagen Type III Alpha 1 (COL3a1), ELISA Kit

Full Product Name

Mouse Collagen Type III Alpha 1 (COL3a1) ELISA Kit

Product Gene Name

COL3a1 elisa kit

Product Synonym Names

COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Mouse serum, plasma, tissue homogenates or other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

< 14.5pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of COL3a1. No significant cross-reactivity or interference between COL3a1 and analogues was observed.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL3a1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL3a1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

138,943 Da

3D Structure

ModBase 3D Structure for P08121

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of COL3a1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL3a1 purchase

MBS457004 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type III Alpha 1 (COL3a1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL3a1. The ELISA analytical biochemical technique of the MBS457004 kit is based on COL3a1 antibody-COL3a1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL3a1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL3a1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for COL3a1. It may not necessarily be applicable to this product.

NCBI GI #

226423933

NCBI GeneID

12825

NCBI Accession #

NP_034060.2

UniProt Primary Accession #

P08121

UniProt Secondary Accession #

Q61429; Q9CRN7

UniProt Related Accession #

P08121

GenBank Nucleotide #

NM_009930.2

NCBI Official Full Name

collagen alpha-1(III) chain preproprotein

NCBI Official Synonym Full Names

collagen, type III, alpha 1

NCBI Official Symbol

Col3a1

NCBI Official Synonym Symbols

Tsk2; Tsk-2; Col3a-1; AW550625

UniProt Protein Name

Collagen alpha-1(III) chain

Protein Family

Collagen

UniProt Gene Name

Col3a1

NCBI Protein Information

collagen alpha-1(III) chain

NCBI Summary for COL3a1

This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015]

UniProt Comments for COL3a1

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Research Articles on COL3a1

1. osthole could inhibit the collagen I and III expressions and their ratio in CFs treated with TGF-beta1 via Smad signaling pathway, which might be one of its anti-fibrotic action mechanisms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with COL3a1 elisa kit

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Collagen Adhesion Via GPVI Pathway antibodies	Collagen Adhesion Via GPVI Pathway Diagram
Collagen Adhesion Via Gp IV Pathway antibodies	Collagen Adhesion Via Gp IV Pathway Diagram
Collagen Adhesion Via Alpha 2 Beta 1 Glycoprotein Pathway antibodies	Collagen Adhesion Via Alpha 2 Beta 1 Glycoprotein Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with COL3a1 elisa kit

Disease Name	Pubmed Publications
OSTEOGENESIS IMPERFECTA, TYPE I Antibodies	>206 publications with COL3a1 and OSTEOGENESIS IMPERFECTA, TYPE I
Skin Diseases Antibodies	>110 publications with COL3a1 and Skin Diseases
Neoplasms Antibodies	>109 publications with COL3a1 and Neoplasms
Fibrosis Antibodies	>96 publications with COL3a1 and Fibrosis
Osteoporosis Antibodies	>79 publications with COL3a1 and Osteoporosis
Fibrosarcoma Antibodies	>70 publications with COL3a1 and Fibrosarcoma
Skin Neoplasms Antibodies	>60 publications with COL3a1 and Skin Neoplasms
Cardiovascular Diseases Antibodies	>54 publications with COL3a1 and Cardiovascular Diseases
Bone Resorption Antibodies	>28 publications with COL3a1 and Bone Resorption
Inflammation Antibodies	>27 publications with COL3a1 and Inflammation

Organs/Tissues associated with COL3a1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>368 publications with COL3a1 and Bone
Skin Antibodies	>168 publications with COL3a1 and Skin
Connective Tissue Antibodies	>153 publications with COL3a1 and Connective Tissue
Joint Antibodies	>53 publications with COL3a1 and Joint
Lung Antibodies	>39 publications with COL3a1 and Lung
Embryonic Tissue Antibodies	>24 publications with COL3a1 and Embryonic Tissue
Kidney Antibodies	>22 publications with COL3a1 and Kidney
Prostate Antibodies	>8 publications with COL3a1 and Prostate
Uterus Antibodies	>5 publications with COL3a1 and Uterus
Intestine Antibodies	>3 publications with COL3a1 and Intestine