Phosphodiesterase 3a, Cgmp Inhibited (PDE3A) ELISA Kit for Human

Name: Human PDE3A ELISA Kit; Phosphodiesterase 3a, Cgmp Inhibited; MyBioSource
Brand: MyBioSource
SKU: MBS457124
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS457124

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphodiesterase 3A, cGMP Inhibited (PDE3A), ELISA Kit

Full Product Name

Human Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit

Product Gene Name

PDE3A elisa kit

Product Synonym Names

CGI-PDE; Cyclic GMP-inhibited phosphodiesterase A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human tissue homogenates or other biological fluids

Detection Range

0.312-20ng/mL

Sensitivity

< 0.101ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of PDE3A. No significant cross-reactivity or interference between PDE3A and analogues was observed.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDE3A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDE3A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

124,979 Da

OMIM

M91667 mRNA

3D Structure

ModBase 3D Structure for Q14432

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PDE3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE3A purchase

MBS457124 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDE3A. The ELISA analytical biochemical technique of the MBS457124 kit is based on PDE3A antibody-PDE3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDE3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDE3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PDE3A. It may not necessarily be applicable to this product.

NCBI GI #

70608155

NCBI GeneID

5139

NCBI Accession #

NP_000912.3

UniProt Primary Accession #

Q14432

UniProt Secondary Accession #

O60865; Q13348; Q17RD1

UniProt Related Accession #

Q14432

GenBank Nucleotide #

NM_000921.4

NCBI Official Full Name

cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 1

NCBI Official Synonym Full Names

phosphodiesterase 3A

NCBI Official Symbol

PDE3A

NCBI Official Synonym Symbols

HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A

UniProt Protein Name

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

UniProt Synonym Protein Names

Cyclic GMP-inhibited phosphodiesterase A; CGI-PDE A

Protein Family

cGMP-inhibited 3',5'-cyclic phosphodiesterase

UniProt Gene Name

PDE3A

UniProt Synonym Gene Names

CGI-PDE A

NCBI Protein Information

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

NCBI Summary for PDE3A

This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for PDE3A

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.

Research Articles on PDE3A

1. Vulnerability to antineoplastic phosphodiesterase inhibitors inhibitors has been associated with co-expression of PDE3A and SLFN12 in various tumor types.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PDE3A elisa kit

Products by Pathway	Pathway Diagram
Formation Of Platelet Plug Pathway antibodies	Formation Of Platelet Plug Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Nitric Oxide Stimulates Guanylate Cyclase Pathway antibodies	Nitric Oxide Stimulates Guanylate Cyclase Pathway Diagram
Platelet Activation Pathway antibodies	Platelet Activation Pathway Diagram
Platelet Homeostasis Pathway antibodies	Platelet Homeostasis Pathway Diagram
Progesterone-mediated Oocyte Maturation Pathway antibodies	Progesterone-mediated Oocyte Maturation Pathway Diagram

Diseases associated with PDE3A elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>14 publications with PDE3A and Cardiovascular Diseases
Neoplasms Antibodies	>11 publications with PDE3A and Neoplasms
Heart Failure Antibodies	>10 publications with PDE3A and Heart Failure
Obesity Antibodies	>5 publications with PDE3A and Obesity
Hypertension Antibodies	>3 publications with PDE3A and Hypertension
Insulin Resistance Antibodies	>2 publications with PDE3A and Insulin Resistance
Coronary Artery Disease Antibodies	>2 publications with PDE3A and Coronary Artery Disease
Cardiomegaly Antibodies	>2 publications with PDE3A and Cardiomegaly
Diabetes Mellitus, Experimental Antibodies	>2 publications with PDE3A and Diabetes Mellitus, Experimental
Fibrosis Antibodies	>2 publications with PDE3A and Fibrosis

Organs/Tissues associated with PDE3A elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>43 publications with PDE3A and Muscle
Vascular Antibodies	>30 publications with PDE3A and Vascular
Brain Antibodies	>7 publications with PDE3A and Brain
Liver Antibodies	>6 publications with PDE3A and Liver
Bone Antibodies	>5 publications with PDE3A and Bone
Kidney Antibodies	>5 publications with PDE3A and Kidney
Connective Tissue Antibodies	>4 publications with PDE3A and Connective Tissue
Embryonic Tissue Antibodies	>4 publications with PDE3A and Embryonic Tissue
Placenta Antibodies	>3 publications with PDE3A and Placenta