Brain Finger (BFP) ELISA Kit for Mouse

Name: Mouse BFP ELISA Kit; Brain Finger; MyBioSource
Brand: MyBioSource
SKU: MBS457903
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS457903

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BFP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BFP. Next,… More details

$530 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Brain Finger Protein (BFP), ELISA Kit

Full Product Name

Mouse Brain Finger Protein (BFP) ELISA Kit

Product Gene Name

BFP elisa kit

Product Synonym Names

RNF179; RNF112; Ring Finger Protein 179; Ring Finger Protein 112

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Tissue Homogenates or Other Biological Fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.066ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

74,115 Da

3D Structure

ModBase 3D Structure for Q96DY5

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of BFP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BFP purchase

MBS457903 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Brain Finger Protein (BFP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BFP. The ELISA analytical biochemical technique of the MBS457903 kit is based on BFP antibody-BFP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BFP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BFP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BFP. It may not necessarily be applicable to this product.

NCBI GI #

597518007

NCBI GeneID

22671

NCBI Accession #

NP_001277953.1

UniProt Primary Accession #

Q96DY5

UniProt Secondary Accession #

Q3TNS6; Q5NCN2; Q8JZT5; Q9Z328

UniProt Related Accession #

Q96DY5

GenBank Nucleotide #

NM_001291024.1

NCBI Official Full Name

RING finger protein 112 isoform 2

NCBI Official Synonym Full Names

ring finger protein 112

NCBI Official Symbol

Rnf112

NCBI Official Synonym Symbols

bfp; ZNF179; Zfp179; neurolastin

UniProt Protein Name

RING finger protein 112

UniProt Synonym Protein Names

Brain finger protein; Neurolastin

UniProt Gene Name

Rnf112

UniProt Synonym Gene Names

Bfp; Znf179

NCBI Protein Information

RING finger protein 112

UniProt Comments for BFP

E3 ubiquitin-protein ligase that plays an important role in neuronal differentiation, including neurogenesis and gliogenesis, during brain development. During embryonic development initiates neuronal differentiation by inducing cell cycle arrest at the G0/G1 phase through up-regulation of cell-cycle regulatory proteins (PubMed:21566658, PubMed:28684796). Plays a role not only in the fetal period during the development of the nervous system, but also in the adult brain, where it is involved in the maintenance of neural functions and protection of the nervous tissue cells from oxidative stress-induced damage (PubMed:27918959, PubMed:26792191, PubMed:26951452). Exhibits GTPase and E3 ubiquitin-protein ligase activities. Regulates dendritic spine density and synaptic neurotransmission; its ability to hydrolyze GTP is involved in the maintenance of dendritic spine density (PubMed:26212327).

Research Articles on BFP

1. Rnf112 protein is expressed in most brain areas and mainly in neurons; deletion of both Rnf112 alleles resulted in embryonic lethality associated with defects in embryonic vasculature; results provide evidence for the important functions of Rnf112 in embryo development and in adult brain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with BFP elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with BFP and Brain