Caspase 8 (CASP8) ELISA Kit for Mouse

Name: Mouse CASP8 ELISA Kit; Caspase 8; MyBioSource
Brand: MyBioSource
SKU: MBS458135
Price: 470 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS458135

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CASP8.… More details

$470 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Caspase 8 (CASP8), ELISA Kit

Full Product Name

Mouse Caspase 8 (CASP8) ELISA Kit

Product Gene Name

CASP8 elisa kit

Product Synonym Names

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.069ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

55,357 Da

3D Structure

ModBase 3D Structure for O89110

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of CASP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CASP8 purchase

MBS458135 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caspase 8 (CASP8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CASP8. The ELISA analytical biochemical technique of the MBS458135 kit is based on CASP8 antibody-CASP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CASP8. It may not necessarily be applicable to this product.

NCBI GI #

122056578

NCBI GeneID

12370

NCBI Accession #

NP_001073595.1

UniProt Primary Accession #

O89110

UniProt Secondary Accession #

O35669

UniProt Related Accession #

O89110

GenBank Nucleotide #

NM_001080126.1

NCBI Official Full Name

caspase-8 isoform 1

NCBI Official Synonym Full Names

caspase 8

NCBI Official Symbol

Casp8

NCBI Official Synonym Symbols

MACH; Mch5; FLICE; CASP-8

UniProt Protein Name

Caspase-8

Protein Family

Caspase

UniProt Gene Name

Casp8

UniProt Synonym Gene Names

CASP-8

NCBI Protein Information

caspase-8

NCBI Summary for CASP8

This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

UniProt Comments for CASP8

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

Research Articles on CASP8

1. Results illustrate the temporal and spatial activation of caspase-8 and -3 in microglia/macrophages occurring upon ischemic stroke and suggest that the expression of these caspases could be used in neuropathological diagnostic work.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CASP8 elisa kit

Reference Product	PubMed Publications
Fas elisa kit	>3218 publications with CASP8 and Fas
Fadd elisa kit	>2325 publications with CASP8 and Fadd
Cflar elisa kit	>921 publications with CASP8 and Cflar
Fasl elisa kit	>862 publications with CASP8 and Fasl
Tnfsf10 elisa kit	>778 publications with CASP8 and Tnfsf10
Bcl2 elisa kit	>242 publications with CASP8 and Bcl2
Tnfrsf10b elisa kit	>193 publications with CASP8 and Tnfrsf10b
Tradd elisa kit	>178 publications with CASP8 and Tradd
Ripk1 elisa kit	>172 publications with CASP8 and Ripk1
Traf2 elisa kit	>116 publications with CASP8 and Traf2

Pathways associated with CASP8 elisa kit

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Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis - Multiple Species Pathway antibodies	Apoptosis - Multiple Species Pathway Diagram
Apoptosis - Multiple Species Pathway antibodies	Apoptosis - Multiple Species Pathway Diagram

Diseases associated with CASP8 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>2868 publications with CASP8 and Necrosis
Inflammation Antibodies	>640 publications with CASP8 and Inflammation
Adenocarcinoma Antibodies	>619 publications with CASP8 and Adenocarcinoma
Nervous System Diseases Antibodies	>515 publications with CASP8 and Nervous System Diseases
Breast Neoplasms Antibodies	>485 publications with CASP8 and Breast Neoplasms
Liver Diseases Antibodies	>475 publications with CASP8 and Liver Diseases
Colorectal Neoplasms Antibodies	>359 publications with CASP8 and Colorectal Neoplasms
Lung Neoplasms Antibodies	>342 publications with CASP8 and Lung Neoplasms
Carcinoma, Hepatocellular Antibodies	>295 publications with CASP8 and Carcinoma, Hepatocellular
Neoplasms, Experimental Antibodies	>274 publications with CASP8 and Neoplasms, Experimental

Organs/Tissues associated with CASP8 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1674 publications with CASP8 and Blood
Other Antibodies	>1499 publications with CASP8 and Other
Liver Antibodies	>806 publications with CASP8 and Liver
Brain Antibodies	>639 publications with CASP8 and Brain
Lung Antibodies	>626 publications with CASP8 and Lung
Bone Antibodies	>344 publications with CASP8 and Bone
Kidney Antibodies	>315 publications with CASP8 and Kidney
Skin Antibodies	>247 publications with CASP8 and Skin
Bone Marrow Antibodies	>204 publications with CASP8 and Bone Marrow
Embryonic Tissue Antibodies	>118 publications with CASP8 and Embryonic Tissue