Human DEFb126 ELISA Kit – Cat# MBS458823

Defensin Beta 126 (DEFb126) ELISA Kit for Human

Name: Human DEFb126 ELISA Kit; Defensin Beta 126; MyBioSource
Brand: MyBioSource
SKU: MBS458823
Price: 575 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS458823

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEFb126. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$575 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Defensin Beta 126 (DEFb126), ELISA Kit

Full Product Name

Human Defensin Beta 126 (DEFb126) ELISA Kit

Product Gene Name

DEFb126 elisa kit

Product Synonym Names

ESP13.2; C20orf8; DEFB-26; Epididymal secretory protein 13.2

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.

Detection Range

78.1-5000pg/mL

Sensitivity

34pg/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

12,174 Da

3D Structure

ModBase 3D Structure for Q9BYW3

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of DEFb126 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEFb126 purchase

MBS458823 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Beta 126 (DEFb126) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DEFb126. The ELISA analytical biochemical technique of the MBS458823 kit is based on DEFb126 antibody-DEFb126 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFb126 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFb126. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DEFb126. It may not necessarily be applicable to this product.

NCBI GI #

13624333

NCBI GeneID

81623

NCBI Accession #

NP_112193.1

UniProt Primary Accession #

Q9BYW3

UniProt Secondary Accession #

Q562G3; Q9H1M5

UniProt Related Accession #

Q9BYW3

GenBank Nucleotide #

NM_030931.3

NCBI Official Full Name

beta-defensin 126 preproprotein

NCBI Official Synonym Full Names

defensin beta 126

NCBI Official Symbol

DEFB126

NCBI Official Synonym Symbols

HBD26; DEFB26; hBD-26; C20orf8; DEFB-26; bA530N10.1

UniProt Protein Name

Beta-defensin 126

UniProt Synonym Protein Names

Beta-defensin 26; DEFB-26; Defensin, beta 126; Epididymal secretory protein 13.2; ESP13.2; HBD26

Protein Family

Beta-defensin

UniProt Gene Name

DEFB126

UniProt Synonym Gene Names

C20orf8; DEFB26; DEFB-26; ESP13.2

NCBI Protein Information

beta-defensin 126

NCBI Summary for DEFb126

Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. The encoded protein is highly similar to an epididymal-specific secretory protein (ESP13.2) from cynomolgus monkey. Mutation of this gene is associated with impaired sperm function. [provided by RefSeq, Nov 2014]

UniProt Comments for DEFb126

Highly glycosylated atypical beta-defensin involved in several aspects of sperm function. Facilitates sperm transport in the female reproductive tract and contributes to sperm protection against immunodetection; both functions are probably implicating the negative surface charge provided by its O-linked oligosaccharides in the sperm glycocalyx. Involved in binding of sperm to oviductal epithelial cells to form a sperm reservoir until ovulation. Release from the sperm surface during capacitation and ovaluation by an elevation of oviductal fluid pH is unmasking other surface components and allows sperm to penetrate the cumulus matrix and bind to the zona pellucida of the oocyte (). In vitro has antimicrobial activity and may inhibit LPS-mediated inflammation (PubMed:19373462, PubMed:23229569).

Research Articles on DEFb126

1. Data indicate a significant contribution of the frame-shift polymorphism of defensin beta 126 (DEFB126) rs11467497 to male infertility.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DEFb126 elisa kit

Products by Pathway	Pathway Diagram
Beta Defensins Pathway antibodies	Beta Defensins Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Organs/Tissues associated with DEFb126 elisa kit

Organ/Tissue Name	Pubmed Publications
Epididymis Antibodies	>36 publications with DEFb126 and Epididymis
Testis Antibodies	>3 publications with DEFb126 and Testis