Dysferlin (DYSF) ELISA Kit for Human

Name: Human DYSF ELISA Kit; Dysferlin; MyBioSource
Brand: MyBioSource
SKU: MBS458957
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS458957

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DYSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$540 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Dysferlin (DYSF), ELISA Kit

Full Product Name

Human Dysferlin (DYSF) ELISA Kit

Product Gene Name

DYSF elisa kit

Product Synonym Names

FER1L1; LGMD2B; Dysferlin,Limb Girdle Muscular Dystrophy 2B(Autosomal Recessive); Fer-1-like protein 1; Dystrophy-associated fer-1-like protein

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human tissue homogenates or other biological fluids

Detection Range

0.312-20ng/mL

Sensitivity

< 0.141ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of DYSF. No significant cross-reactivity or interference between DYSF and analogues was observed.

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DYSF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DYSF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

222,315 Da

OMIM

253601

3D Structure

ModBase 3D Structure for O75923

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of DYSF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DYSF purchase

MBS458957 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dysferlin (DYSF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DYSF. The ELISA analytical biochemical technique of the MBS458957 kit is based on DYSF antibody-DYSF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DYSF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DYSF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DYSF. It may not necessarily be applicable to this product.

NCBI GI #

195976821

NCBI GeneID

8291

NCBI Accession #

NP_001123927.1

UniProt Primary Accession #

O75923

UniProt Secondary Accession #

A0FK00; B1PZ70; B1PZ71; B1PZ72; B1PZ73; B1PZ74; B1PZ75; B1PZ76; B1PZ77; B1PZ78; B1PZ79

UniProt Related Accession #

O75923

GenBank Nucleotide #

NM_001130455.1

NCBI Official Full Name

dysferlin isoform 2

NCBI Official Synonym Full Names

dysferlin

NCBI Official Symbol

DYSF

NCBI Official Synonym Symbols

MMD1; FER1L1; LGMD2B

UniProt Protein Name

Dysferlin

UniProt Synonym Protein Names

Dystrophy-associated fer-1-like protein; Fer-1-like protein 1

Protein Family

Dysferlin

UniProt Gene Name

DYSF

UniProt Synonym Gene Names

FER1L1

NCBI Protein Information

dysferlin

NCBI Summary for DYSF

The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2008]

UniProt Comments for DYSF

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress ().

Research Articles on DYSF

1. Immunofluorescence demonstrated that the percentage of complex I- and complex IV-deficient fibres was higher in patients with DYSF mutations than in age-matched controls. No clonally expanded mtDNA deletions were detected using long-range PCR in any of the analysed muscle fibres. Complex I and complex IV deficiency is higher in patients than age matched controls but patients do not have rearrangements of the mtDNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DYSF elisa kit

Reference Product	PubMed Publications
CAPN3 elisa kit	>19 publications with DYSF and CAPN3
CAV3 elisa kit	>13 publications with DYSF and CAV3
ANXA2 elisa kit	>1 publications with DYSF and ANXA2

Diseases associated with DYSF elisa kit

Disease Name	Pubmed Publications
Miyoshi myopathy Antibodies	>123 publications with DYSF and Miyoshi myopathy
Disease Models, Animal Antibodies	>33 publications with DYSF and Disease Models, Animal
Inflammation Antibodies	>30 publications with DYSF and Inflammation
Necrosis Antibodies	>16 publications with DYSF and Necrosis
Hypertrophy Antibodies	>7 publications with DYSF and Hypertrophy
Myopathy, Distal, with Anterior Tibial Onset Antibodies	>7 publications with DYSF and Myopathy, Distal, with Anterior Tibial Onset
Neoplasms Antibodies	>5 publications with DYSF and Neoplasms
Limb-girdle muscular dystrophy, type 2B Antibodies	>5 publications with DYSF and Limb-girdle muscular dystrophy, type 2B
Congenital Abnormalities Antibodies	>3 publications with DYSF and Congenital Abnormalities
Kidney Diseases Antibodies	>2 publications with DYSF and Kidney Diseases

Organs/Tissues associated with DYSF elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>313 publications with DYSF and Muscle
Blood Antibodies	>53 publications with DYSF and Blood
Heart Antibodies	>17 publications with DYSF and Heart
Nerve Antibodies	>16 publications with DYSF and Nerve
Brain Antibodies	>11 publications with DYSF and Brain
Embryonic Tissue Antibodies	>8 publications with DYSF and Embryonic Tissue
Vascular Antibodies	>6 publications with DYSF and Vascular
Placenta Antibodies	>5 publications with DYSF and Placenta
Lung Antibodies	>4 publications with DYSF and Lung
Kidney Antibodies	>4 publications with DYSF and Kidney