E2F Transcription Factor 3 (E2F3) ELISA Kit for Human

Name: Human E2F3 ELISA Kit; E2F Transcription Factor 3; MyBioSource
Brand: MyBioSource
SKU: MBS458962
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS458962

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to E2F3.… More details

$540 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

E2F Transcription Factor 3 (E2F3), ELISA Kit

Full Product Name

Human E2F Transcription Factor 3 (E2F3) ELISA Kit

Product Gene Name

E2F3 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Tissue Homogenates or Other Biological Fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.072ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

37,010 Da

OMIM

600427

3D Structure

ModBase 3D Structure for O00716

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of E2F3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for E2F3 purchase

MBS458962 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the E2F Transcription Factor 3 (E2F3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing E2F3. The ELISA analytical biochemical technique of the MBS458962 kit is based on E2F3 antibody-E2F3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect E2F3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, E2F3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for E2F3. It may not necessarily be applicable to this product.

NCBI GI #

340523141

NCBI GeneID

1871

NCBI Accession #

NP_001230005.1

UniProt Primary Accession #

O00716

UniProt Secondary Accession #

Q15000; Q68DT0; Q9BZ44

UniProt Related Accession #

O00716

GenBank Nucleotide #

NM_001243076.2

NCBI Official Full Name

transcription factor E2F3 isoform 2

NCBI Official Synonym Full Names

E2F transcription factor 3

NCBI Official Symbol

E2F3

NCBI Official Synonym Symbols

E2F-3

UniProt Protein Name

Transcription factor E2F3

Protein Family

Transcription factor

UniProt Gene Name

E2F3

UniProt Synonym Gene Names

KIAA0075; E2F-3

NCBI Protein Information

transcription factor E2F3

NCBI Summary for E2F3

This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

UniProt Comments for E2F3

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters ().

Research Articles on E2F3

1. The increased expression of miR-503-5p significantly reduced the expressions of E2F transcription factor 3 (E2F3) mRNA and retinoblastoma protein (Rb)/E2F signaling pathway mRNA in bladder cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with E2F3 elisa kit

Reference Product	PubMed Publications
RB1 elisa kit	>22 publications with E2F3 and RB1
TFDP1 elisa kit	>15 publications with E2F3 and TFDP1
RBL1 elisa kit	>12 publications with E2F3 and RBL1
YY1 elisa kit	>4 publications with E2F3 and YY1
CCNA2 elisa kit	>3 publications with E2F3 and CCNA2
CCNE1 elisa kit	>3 publications with E2F3 and CCNE1
TFDP2 elisa kit	>3 publications with E2F3 and TFDP2
TFE3 elisa kit	>3 publications with E2F3 and TFE3
RYBP elisa kit	>1 publications with E2F3 and RYBP

Pathways associated with E2F3 elisa kit

Products by Pathway	Pathway Diagram
Assembly Of The Pre-replicative Complex Pathway antibodies	Assembly Of The Pre-replicative Complex Pathway Diagram
Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies	Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
CDC6 Association With The ORC:origin Complex Pathway antibodies	CDC6 Association With The ORC:origin Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram

Diseases associated with E2F3 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>56 publications with E2F3 and Carcinoma
Breast Neoplasms Antibodies	>20 publications with E2F3 and Breast Neoplasms
Adenocarcinoma Antibodies	>19 publications with E2F3 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>17 publications with E2F3 and Neoplasms, Experimental
Ovarian Neoplasms Antibodies	>16 publications with E2F3 and Ovarian Neoplasms
Ovarian Diseases Antibodies	>15 publications with E2F3 and Ovarian Diseases
Lung Neoplasms Antibodies	>15 publications with E2F3 and Lung Neoplasms
Urinary Bladder Diseases Antibodies	>14 publications with E2F3 and Urinary Bladder Diseases
Urinary Bladder Neoplasms Antibodies	>14 publications with E2F3 and Urinary Bladder Neoplasms
Carcinoma, Hepatocellular Antibodies	>13 publications with E2F3 and Carcinoma, Hepatocellular

Organs/Tissues associated with E2F3 elisa kit

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>27 publications with E2F3 and Prostate
Lung Antibodies	>21 publications with E2F3 and Lung
Eye Antibodies	>20 publications with E2F3 and Eye
Blood Antibodies	>18 publications with E2F3 and Blood
Brain Antibodies	>15 publications with E2F3 and Brain
Embryonic Tissue Antibodies	>15 publications with E2F3 and Embryonic Tissue
Heart Antibodies	>11 publications with E2F3 and Heart
Bone Antibodies	>11 publications with E2F3 and Bone
Kidney Antibodies	>8 publications with E2F3 and Kidney
Skin Antibodies	>7 publications with E2F3 and Skin