Elastase 2, Neutrophil (ELA2) ELISA Kit for Mouse

Name: Mouse ELA2 ELISA Kit; Elastase 2, Neutrophil; MyBioSource
Brand: MyBioSource
SKU: MBS459032
Price: 510 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459032

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELA2.… More details

$510 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Elastase 2, Neutrophil (ELA2), ELISA Kit

Full Product Name

Mouse Elastase 2, Neutrophil (ELA2) ELISA Kit

Product Gene Name

ELA2 elisa kit

Product Synonym Names

ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma or Other Biological Fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.063ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

28,648 Da

3D Structure

ModBase 3D Structure for Q3UP87

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ELA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELA2 purchase

MBS459032 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Elastase 2, Neutrophil (ELA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ELA2. The ELISA analytical biochemical technique of the MBS459032 kit is based on ELA2 antibody-ELA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ELA2. It may not necessarily be applicable to this product.

NCBI GI #

114052444

NCBI GeneID

50701

NCBI Accession #

NP_056594.2

UniProt Primary Accession #

Q3UP87

UniProt Secondary Accession #

Q61515; A6H698

UniProt Related Accession #

Q3UP87

GenBank Nucleotide #

NM_015779.2

NCBI Official Full Name

neutrophil elastase

NCBI Official Synonym Full Names

elastase, neutrophil expressed

NCBI Official Symbol

Elane

NCBI Official Synonym Symbols

NE; Ela2; F430011M15Rik

UniProt Protein Name

Neutrophil elastase

UniProt Synonym Protein Names

Elastase-2; Leukocyte elastase

UniProt Gene Name

Elane

UniProt Synonym Gene Names

Ela2

NCBI Protein Information

neutrophil elastase

NCBI Summary for ELA2

This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]

UniProt Comments for ELA2

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis ().

Research Articles on ELA2

1. the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ELA2 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with ELA2 elisa kit

Disease Name	Pubmed Publications
Neutropenia Antibodies	>53 publications with ELA2 and Neutropenia
Cyclic neutropenia Antibodies	>12 publications with ELA2 and Cyclic neutropenia
Inflammation Antibodies	>8 publications with ELA2 and Inflammation
Lung Diseases Antibodies	>5 publications with ELA2 and Lung Diseases
Neutropenia, Severe Congenital, Autosomal Dominant 1 Antibodies	>5 publications with ELA2 and Neutropenia, Severe Congenital, Autosomal Dominant 1
Immune System Diseases Antibodies	>4 publications with ELA2 and Immune System Diseases
Pneumonia Antibodies	>4 publications with ELA2 and Pneumonia
Disease Models, Animal Antibodies	>2 publications with ELA2 and Disease Models, Animal
Weight Loss Antibodies	>2 publications with ELA2 and Weight Loss
Chromosome Aberrations Antibodies	>1 publications with ELA2 and Chromosome Aberrations

Organs/Tissues associated with ELA2 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>40 publications with ELA2 and Blood
Bone Marrow Antibodies	>11 publications with ELA2 and Bone Marrow