Elastase 3a (ELA3A) ELISA Kit for Human

Name: Human ELA3A ELISA Kit; Elastase 3a; MyBioSource
Brand: MyBioSource
SKU: MBS459034
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459034

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELA3A.… More details

$540 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Elastase 3A (ELA3A), ELISA Kit

Full Product Name

Human Elastase 3A (ELA3A) ELISA Kit

Product Gene Name

ELA3A elisa kit

Product Synonym Names

CELA3A; ELA3; Chymotrypsin-Like Elastase Family Member 3A; Protease E

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenates or Other Biological Fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.113ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

29,489 Da

3D Structure

ModBase 3D Structure for P09093

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ELA3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELA3A purchase

MBS459034 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Elastase 3A (ELA3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ELA3A. The ELISA analytical biochemical technique of the MBS459034 kit is based on ELA3A antibody-ELA3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ELA3A. It may not necessarily be applicable to this product.

NCBI GI #

236460050

NCBI GeneID

10136

NCBI Accession #

NP_005738.4

UniProt Primary Accession #

P09093

UniProt Secondary Accession #

Q9BRW4; B1AQ53

UniProt Related Accession #

P09093

GenBank Nucleotide #

NM_005747.4

NCBI Official Full Name

chymotrypsin-like elastase family member 3A preproprotein

NCBI Official Synonym Full Names

chymotrypsin like elastase family member 3A

NCBI Official Symbol

CELA3A

NCBI Official Synonym Symbols

ELA3; ELA3A

UniProt Protein Name

Chymotrypsin-like elastase family member 3A

UniProt Synonym Protein Names

Elastase IIIA; Elastase-3A; Protease E

UniProt Gene Name

CELA3A

UniProt Synonym Gene Names

ELA3; ELA3A

NCBI Protein Information

chymotrypsin-like elastase family member 3A

NCBI Summary for ELA3A

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]

UniProt Comments for ELA3A

Efficient protease with alanine specificity but only little elastolytic activity.

Research Articles on ELA3A

1. Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ELA3A elisa kit

Reference Product	PubMed Publications
CELA2A elisa kit	>2 publications with ELA3A and CELA2A
CELA2B elisa kit	>1 publications with ELA3A and CELA2B
CPA2 elisa kit	>1 publications with ELA3A and CPA2
CPB1 elisa kit	>1 publications with ELA3A and CPB1
CPA1 elisa kit	>1 publications with ELA3A and CPA1

Pathways associated with ELA3A elisa kit

Products by Pathway	Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram

Organs/Tissues associated with ELA3A elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>4 publications with ELA3A and Pancreas