Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit for Human

Name: Human ENPP1 ELISA Kit; Ectonucleotide Pyrophosphatase/Phosphodiesterase 1; MyBioSource
Brand: MyBioSource
SKU: MBS459065
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459065

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENPP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ENPP1.… More details

$540 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), ELISA Kit

Full Product Name

Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit

Product Gene Name

ENPP1 elisa kit

Product Synonym Names

M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1; Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenates or Other Biological Fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.062ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

104,924 Da

OMIM

125853

3D Structure

ModBase 3D Structure for P22413

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ENPP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENPP1 purchase

MBS459065 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENPP1. The ELISA analytical biochemical technique of the MBS459065 kit is based on ENPP1 antibody-ENPP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENPP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENPP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ENPP1. It may not necessarily be applicable to this product.

NCBI GI #

170650661

NCBI GeneID

5167

NCBI Accession #

NP_006199.2

UniProt Primary Accession #

P22413

UniProt Secondary Accession #

Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3

UniProt Related Accession #

P22413

GenBank Nucleotide #

NM_006208.2

NCBI Official Full Name

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

NCBI Official Synonym Full Names

ectonucleotide pyrophosphatase/phosphodiesterase 1

NCBI Official Symbol

ENPP1

NCBI Official Synonym Symbols

M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1

UniProt Protein Name

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

UniProt Synonym Protein Names

Nucleotide diphosphataseCurated

Protein Family

Ectonucleotide pyrophosphatase/phosphodiesterase family

UniProt Gene Name

ENPP1

UniProt Synonym Gene Names

M6S1; NPPS; PC1; PDNP1; NPPase

NCBI Protein Information

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

NCBI Summary for ENPP1

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

UniProt Comments for ENPP1

By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function.

Research Articles on ENPP1

1. This study identifies the genetic variant rs9373000 as a potentially causal variant for mandibular condyle geometry variation for patients presenting with dento-facial deformities.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ENPP1 elisa kit

Reference Product	PubMed Publications
NT5E elisa kit	>5 publications with ENPP1 and NT5E
CD38 elisa kit	>1 publications with ENPP1 and CD38

Pathways associated with ENPP1 elisa kit

Products by Pathway	Pathway Diagram
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective BTD Causes Biotidinase Deficiency Pathway antibodies	Defective BTD Causes Biotidinase Deficiency Pathway Diagram
Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies	Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies	Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies	Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies	Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies	Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies	Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies	Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with ENPP1 elisa kit

Disease Name	Pubmed Publications
Diabetes Mellitus, Type 2 Antibodies	>64 publications with ENPP1 and Diabetes Mellitus, Type 2
Obesity Antibodies	>55 publications with ENPP1 and Obesity
Arterial calcification of infancy Antibodies	>32 publications with ENPP1 and Arterial calcification of infancy
Kidney Diseases Antibodies	>23 publications with ENPP1 and Kidney Diseases
Congenital Abnormalities Antibodies	>16 publications with ENPP1 and Congenital Abnormalities
Disease Models, Animal Antibodies	>16 publications with ENPP1 and Disease Models, Animal
Hypertension Antibodies	>11 publications with ENPP1 and Hypertension
Liver Diseases Antibodies	>9 publications with ENPP1 and Liver Diseases
Inflammation Antibodies	>6 publications with ENPP1 and Inflammation
Hypophosphatemic Rickets, Autosomal Recessive, 2 Antibodies	>5 publications with ENPP1 and Hypophosphatemic Rickets, Autosomal Recessive, 2

Organs/Tissues associated with ENPP1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>57 publications with ENPP1 and Bone
Blood Antibodies	>51 publications with ENPP1 and Blood
Heart Antibodies	>25 publications with ENPP1 and Heart
Muscle Antibodies	>22 publications with ENPP1 and Muscle
Skin Antibodies	>21 publications with ENPP1 and Skin
Kidney Antibodies	>20 publications with ENPP1 and Kidney
Liver Antibodies	>18 publications with ENPP1 and Liver
Connective Tissue Antibodies	>16 publications with ENPP1 and Connective Tissue
Embryonic Tissue Antibodies	>7 publications with ENPP1 and Embryonic Tissue
Pancreas Antibodies	>4 publications with ENPP1 and Pancreas