Growth Differentiation Factor 5 (GDF5) ELISA Kit for Human

Name: Human GDF5 ELISA Kit; Growth Differentiation Factor 5; MyBioSource
Brand: MyBioSource
SKU: MBS459534
Price: 460 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459534

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$460 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Growth Differentiation Factor 5 (GDF5), ELISA Kit

Full Product Name

Human Growth Differentiation Factor 5 (GDF5) ELISA Kit

Product Gene Name

GDF5 elisa kit

Product Synonym Names

CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

< 0.064ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of GDF5. No significant cross-reactivity or interference between GDF5 and analogues was observed.

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

55,411 Da

OMIM

112600

3D Structure

ModBase 3D Structure for P43026

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of GDF5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GDF5 purchase

MBS459534 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Growth Differentiation Factor 5 (GDF5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GDF5. The ELISA analytical biochemical technique of the MBS459534 kit is based on GDF5 antibody-GDF5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GDF5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GDF5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GDF5. It may not necessarily be applicable to this product.

NCBI GI #

611435007

NCBI GeneID

8200

NCBI Accession #

NP_000548.2

UniProt Primary Accession #

P43026

UniProt Secondary Accession #

Q96SB1; E1P5Q2

UniProt Related Accession #

P43026

GenBank Nucleotide #

NM_000557.4

NCBI Official Full Name

growth/differentiation factor 5 preproprotein

NCBI Official Synonym Full Names

growth differentiation factor 5

NCBI Official Symbol

GDF5

NCBI Official Synonym Symbols

OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14

UniProt Protein Name

Growth/differentiation factor 5

UniProt Synonym Protein Names

Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin

Protein Family

Growth/differentiation factor

UniProt Gene Name

GDF5

UniProt Synonym Gene Names

BMP14; CDMP1; GDF-5; BMP-14; CDMP-1; LAP-4; LPS-associated protein 4

NCBI Protein Information

growth/differentiation factor 5

NCBI Summary for GDF5

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis. [provided by RefSeq, Aug 2016]

UniProt Comments for GDF5

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).

Research Articles on GDF5

1. No association has been found between GDF5 +104 T/C promoter polymorphism and osteoarthritis in the Eastern Turkey population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GDF5 elisa kit

Reference Product	PubMed Publications
BMPR1A elisa kit	>41 publications with GDF5 and BMPR1A
BMPR2 elisa kit	>26 publications with GDF5 and BMPR2
BMPR1B elisa kit	>23 publications with GDF5 and BMPR1B
NOG elisa kit	>14 publications with GDF5 and NOG
RUNX2 elisa kit	>10 publications with GDF5 and RUNX2
COL1A1 elisa kit	>8 publications with GDF5 and COL1A1
SMAD5 elisa kit	>7 publications with GDF5 and SMAD5

Pathways associated with GDF5 elisa kit

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Elastic Fibre Formation Pathway antibodies	Elastic Fibre Formation Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram
Molecules Associated With Elastic Fibres Pathway antibodies	Molecules Associated With Elastic Fibres Pathway Diagram
TGF-beta Signaling Pathway antibodies	TGF-beta Signaling Pathway Diagram
TGF-beta Signaling Pathway antibodies	TGF-beta Signaling Pathway Diagram

Diseases associated with GDF5 elisa kit

Disease Name	Pubmed Publications
Osteoarthritis Antibodies	>81 publications with GDF5 and Osteoarthritis
Disease Models, Animal Antibodies	>41 publications with GDF5 and Disease Models, Animal
Brachydactyly type C Antibodies	>15 publications with GDF5 and Brachydactyly type C
Inflammation Antibodies	>14 publications with GDF5 and Inflammation
Parkinson Disease Antibodies	>14 publications with GDF5 and Parkinson Disease
Necrosis Antibodies	>14 publications with GDF5 and Necrosis
Pain Antibodies	>12 publications with GDF5 and Pain
Movement Disorders Antibodies	>11 publications with GDF5 and Movement Disorders
Brain Diseases Antibodies	>11 publications with GDF5 and Brain Diseases
Brachydactyly type A2 Antibodies	>9 publications with GDF5 and Brachydactyly type A2

Organs/Tissues associated with GDF5 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>412 publications with GDF5 and Bone
Connective Tissue Antibodies	>102 publications with GDF5 and Connective Tissue
Embryonic Tissue Antibodies	>52 publications with GDF5 and Embryonic Tissue
Brain Antibodies	>26 publications with GDF5 and Brain
Skin Antibodies	>19 publications with GDF5 and Skin
Eye Antibodies	>10 publications with GDF5 and Eye
Heart Antibodies	>9 publications with GDF5 and Heart
Lung Antibodies	>5 publications with GDF5 and Lung
Prostate Antibodies	>4 publications with GDF5 and Prostate
Uterus Antibodies	>3 publications with GDF5 and Uterus