Glutamate Dehydrogenase 1 (GDH) ELISA Kit for Human

Name: Human GDH ELISA Kit; Glutamate Dehydrogenase 1; MyBioSource
Brand: MyBioSource
SKU: MBS459540
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459540

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GDH. Next,… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Glutamate Dehydrogenase 1 (GDH), ELISA Kit

Full Product Name

Human Glutamate Dehydrogenase 1 (GDH) ELISA Kit

Product Gene Name

GDH elisa kit

Product Synonym Names

GLUD1; Glutamate dehydrogenase 1, mitochondrial

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenates or Other Biological Fluids.

Detection Range

0.625-40U/L

Sensitivity

0.18U/L

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

46,575 Da

OMIM

138130

3D Structure

ModBase 3D Structure for P00367

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of GDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GDH purchase

MBS459540 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutamate Dehydrogenase 1 (GDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GDH. The ELISA analytical biochemical technique of the MBS459540 kit is based on GDH antibody-GDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GDH. It may not necessarily be applicable to this product.

NCBI GI #

974141156

NCBI GeneID

2746

NCBI Accession #

NP_001305829.1

UniProt Primary Accession #

P00367

UniProt Secondary Accession #

Q5TBU3; B3KV55; B4DGN5

UniProt Related Accession #

P00367

GenBank Nucleotide #

NM_001318900.1

NCBI Official Full Name

glutamate dehydrogenase 1, mitochondrial isoform b

NCBI Official Synonym Full Names

glutamate dehydrogenase 1

NCBI Official Symbol

GLUD1

NCBI Official Synonym Symbols

GDH; GDH1; GLUD

UniProt Protein Name

Glutamate dehydrogenase 1, mitochondrial

Protein Family

Glutamate dehydrogenase

UniProt Gene Name

GLUD1

UniProt Synonym Gene Names

GLUD; GDH 1

NCBI Protein Information

glutamate dehydrogenase 1, mitochondrial

NCBI Summary for GDH

This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016]

UniProt Comments for GDH

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate ().

Research Articles on GDH

1. This study showed that SIRT5 supports the anaplerotic entry of glutamine into the TCA cycle in malignant phenotypes of CRC via activating GLUD1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GDH elisa kit

Reference Product	PubMed Publications
GLUL elisa kit	>4 publications with GDH and GLUL
IDH1 elisa kit	>1 publications with GDH and IDH1
GLS elisa kit	>1 publications with GDH and GLS

Pathways associated with GDH elisa kit

Products by Pathway	Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies	Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
D-Glutamine And D-glutamate Metabolism Pathway antibodies	D-Glutamine And D-glutamate Metabolism Pathway Diagram
D-Glutamine And D-glutamate Metabolism Pathway antibodies	D-Glutamine And D-glutamate Metabolism Pathway Diagram

Diseases associated with GDH elisa kit

Disease Name	Pubmed Publications
Hyperinsulinism Antibodies	>74 publications with GDH and Hyperinsulinism
Hyperammonemia Antibodies	>33 publications with GDH and Hyperammonemia
Seizures Antibodies	>15 publications with GDH and Seizures
Hyperinsulinemic hypoglycemia, familial, 6 Antibodies	>7 publications with GDH and Hyperinsulinemic hypoglycemia, familial, 6
Hyperplasia Antibodies	>5 publications with GDH and Hyperplasia
Inflammation Antibodies	>4 publications with GDH and Inflammation
Movement Disorders Antibodies	>4 publications with GDH and Movement Disorders
Liver Diseases Antibodies	>3 publications with GDH and Liver Diseases
Neurobehavioral Manifestations Antibodies	>3 publications with GDH and Neurobehavioral Manifestations
Disease Models, Animal Antibodies	>3 publications with GDH and Disease Models, Animal

Organs/Tissues associated with GDH elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>62 publications with GDH and Brain
Blood Antibodies	>25 publications with GDH and Blood
Pancreas Antibodies	>21 publications with GDH and Pancreas
Liver Antibodies	>18 publications with GDH and Liver
Nerve Antibodies	>16 publications with GDH and Nerve
Testis Antibodies	>9 publications with GDH and Testis
Kidney Antibodies	>7 publications with GDH and Kidney
Placenta Antibodies	>4 publications with GDH and Placenta
Embryonic Tissue Antibodies	>2 publications with GDH and Embryonic Tissue
Vascular Antibodies	>2 publications with GDH and Vascular