Transcription Factor IIA, Polypeptide 1 (GTF2A1) ELISA Kit for Human

Name: Human GTF2A1 ELISA Kit; Transcription Factor IIA, Polypeptide 1; MyBioSource
Brand: MyBioSource
SKU: MBS459758
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459758

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GTF2A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GTF2A1.… More details

$540 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Transcription Factor IIA, Polypeptide 1 (GTF2A1), ELISA Kit

Full Product Name

Human General Transcription Factor IIA, Polypeptide 1 (GTF2A1) ELISA Kit

Product Gene Name

GTF2A1 elisa kit

Product Synonym Names

GTF2-A1; TF2A1; TFIIA; Transcription Initiation Factor IIA Subunit 1; Transcription initiation factor TFIIA 42 kDa subunit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenates or Other Biological Fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.117ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

37,152 Da

OMIM

600520

3D Structure

ModBase 3D Structure for P52655

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of GTF2A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2A1 purchase

MBS459758 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transcription Factor IIA, Polypeptide 1 (GTF2A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GTF2A1. The ELISA analytical biochemical technique of the MBS459758 kit is based on GTF2A1 antibody-GTF2A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GTF2A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GTF2A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2A1. It may not necessarily be applicable to this product.

NCBI GI #

525313614

NCBI GeneID

2957

NCBI Accession #

NP_001265869.1

UniProt Primary Accession #

P52655

UniProt Secondary Accession #

Q3KNQ9

UniProt Related Accession #

P52655

GenBank Nucleotide #

NM_001278940.1

NCBI Official Full Name

transcription initiation factor IIA subunit 1 isoform 3

NCBI Official Synonym Full Names

general transcription factor IIA subunit 1

NCBI Official Symbol

GTF2A1

NCBI Official Synonym Symbols

TF2A1; TFIIA; TFIIAL; TFIIA-42

UniProt Protein Name

Transcription initiation factor IIA subunit 1

UniProt Synonym Protein Names

General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42

Protein Family

Transcription initiation factor

UniProt Gene Name

GTF2A1

UniProt Synonym Gene Names

TF2A1; TFIIA-42

NCBI Protein Information

transcription initiation factor IIA subunit 1

NCBI Summary for GTF2A1

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]

UniProt Comments for GTF2A1

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.

Research Articles on GTF2A1

1. In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GTF2A1 elisa kit

Reference Product	PubMed Publications
GTF2A2 elisa kit	>1 publications with GTF2A1 and GTF2A2
TBP elisa kit	>1 publications with GTF2A1 and TBP

Pathways associated with GTF2A1 elisa kit

Products by Pathway	Pathway Diagram
Basal Transcription Factors Pathway antibodies	Basal Transcription Factors Pathway Diagram
Basal Transcription Factors Pathway antibodies	Basal Transcription Factors Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram
HIV Life Cycle Pathway antibodies	HIV Life Cycle Pathway Diagram
HIV Transcription Initiation Pathway antibodies	HIV Transcription Initiation Pathway Diagram
Late Phase Of HIV Life Cycle Pathway antibodies	Late Phase Of HIV Life Cycle Pathway Diagram
RNA Polymerase II HIV Promoter Escape Pathway antibodies	RNA Polymerase II HIV Promoter Escape Pathway Diagram
RNA Polymerase II Pre-transcription Events Pathway antibodies	RNA Polymerase II Pre-transcription Events Pathway Diagram

Diseases associated with GTF2A1 elisa kit

Disease Name	Pubmed Publications
Ovarian Neoplasms Antibodies	>1 publications with GTF2A1 and Ovarian Neoplasms

Organs/Tissues associated with GTF2A1 elisa kit

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>1 publications with GTF2A1 and Stomach
Testis Antibodies	>1 publications with GTF2A1 and Testis