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Granzyme A (GZMA) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459770

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GZMA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GZMA.… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Granzyme A (GZMA), ELISA Kit
Full Product Name
Human Granzyme A (GZMA) ELISA Kit
Product Gene Name
GZMA elisa kit
Product Synonym Names
CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.054ng/mL
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Molecular Weight
27,109 Da
OMIM
140050
3D Structure
ModBase 3D Structure for P12544
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of GZMA elisa kit

Human GZMA ELISA Kit – Cat# MBS459770

Other Notes

Small volumes of GZMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMA purchase

MBS459770 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Granzyme A (GZMA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GZMA. The ELISA analytical biochemical technique of the MBS459770 kit is based on GZMA antibody-GZMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GZMA. It may not necessarily be applicable to this product.

NCBI GI #
5453676
NCBI GeneID
3001
NCBI Accession #
NP_006135.1
UniProt Primary Accession #
P12544
UniProt Secondary Accession #
Q6IB36; A4PHN1
UniProt Related Accession #
P12544
GenBank Nucleotide #
NM_006144.3
NCBI Official Full Name
granzyme A
NCBI Official Synonym Full Names
granzyme A
NCBI Official Symbol
GZMA
NCBI Official Synonym Symbols
HFSP; CTLA3
UniProt Protein Name
Granzyme A
UniProt Synonym Protein Names
CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF
Protein Family
Granzyme
UniProt Gene Name
GZMA
UniProt Synonym Gene Names
CTLA3; HFSP; H factor; HF
NCBI Protein Information
granzyme A

NCBI Summary for GZMA

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]
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UniProt Comments for GZMA

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
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Research Articles on GZMA

1. granzyme A in human platelets increases with aging

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GZMA elisa kit

 Reference Product  PubMed Publications
 PRF1 elisa kit  >140 publications with GZMA and PRF1
 GZMK elisa kit  >11 publications with GZMA and GZMK
 GNLY elisa kit  >6 publications with GZMA and GNLY
 IL2 elisa kit  >2 publications with GZMA and IL2

Pathways associated with GZMA elisa kit

 Products by Pathway  Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with GZMA elisa kit

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >32 publications with GZMA and Necrosis
 Inflammation Antibodies  >15 publications with GZMA and Inflammation
 Disease Models, Animal Antibodies  >7 publications with GZMA and Disease Models, Animal
 Drug Toxicity Antibodies  >5 publications with GZMA and Drug Toxicity
 Hypersensitivity Antibodies  >5 publications with GZMA and Hypersensitivity
 Gastrointestinal Diseases Antibodies  >5 publications with GZMA and Gastrointestinal Diseases
 Lung Diseases Antibodies  >4 publications with GZMA and Lung Diseases
 Blood Coagulation Disorders Antibodies  >3 publications with GZMA and Blood Coagulation Disorders
 Liver Diseases Antibodies  >3 publications with GZMA and Liver Diseases
 Kidney Diseases Antibodies  >2 publications with GZMA and Kidney Diseases

Organs/Tissues associated with GZMA elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >261 publications with GZMA and Blood
 Lung Antibodies  >10 publications with GZMA and Lung
 Skin Antibodies  >10 publications with GZMA and Skin
 Spleen Antibodies  >9 publications with GZMA and Spleen
 Kidney Antibodies  >9 publications with GZMA and Kidney
 Heart Antibodies  >8 publications with GZMA and Heart
 Intestine Antibodies  >8 publications with GZMA and Intestine
 Uterus Antibodies  >5 publications with GZMA and Uterus
 Lymph Node Antibodies  >5 publications with GZMA and Lymph Node
 Brain Antibodies  >3 publications with GZMA and Brain
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