H2A Histone Family, Member V (H2AFV) ELISA Kit for Human

Name: Human H2AFV ELISA Kit; H2A Histone Family, Member V; MyBioSource
Brand: MyBioSource
SKU: MBS459783
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS459783

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2AFV. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to H2AFV.… More details

$540 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

H2A Histone Family, Member V (H2AFV), ELISA Kit

Full Product Name

Human H2A Histone Family, Member V (H2AFV) ELISA Kit

Product Gene Name

H2AFV elisa kit

Product Synonym Names

H2AV

Species Reactivity

Human

Assay Type

Sandwich

Samples

Tissue Homogenates, Cell Lysates or Other Biological Fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.057ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

6,892 Da

3D Structure

ModBase 3D Structure for Q71UI9

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of H2AFV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H2AFV purchase

MBS459783 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the H2A Histone Family, Member V (H2AFV) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing H2AFV. The ELISA analytical biochemical technique of the MBS459783 kit is based on H2AFV antibody-H2AFV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect H2AFV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, H2AFV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for H2AFV. It may not necessarily be applicable to this product.

NCBI GI #

6912616

NCBI GeneID

94239

NCBI Accession #

NP_036544.1

UniProt Primary Accession #

Q71UI9

UniProt Secondary Accession #

Q59GV8; Q6PK98; A6NFA8; A6NKY0; A6NN01; A8MQC5

UniProt Related Accession #

Q71UI9

GenBank Nucleotide #

NM_012412.4

NCBI Official Full Name

histone H2A.V isoform 1

NCBI Official Synonym Full Names

H2A histone family member V

NCBI Official Symbol

H2AFV

NCBI Official Synonym Symbols

H2AV; H2A.Z-2

UniProt Protein Name

Histone H2A.V

UniProt Synonym Protein Names

H2A.F/Z

Protein Family

Histone

UniProt Gene Name

H2AFV

UniProt Synonym Gene Names

H2AV

NCBI Protein Information

histone H2A.V

NCBI Summary for H2AFV

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene. [provided by RefSeq, Oct 2015]

UniProt Comments for H2AFV

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division ().

Research Articles on H2AFV

1. A mutational analysis revealed that the amino-acid difference at position 38 is at least partially responsible for the structural polymorphism in the L1 loop region of H2A.Z.1 and H2A.Z.2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with H2AFV elisa kit

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Organs/Tissues associated with H2AFV elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with H2AFV and Blood
Muscle Antibodies	>1 publications with H2AFV and Muscle
Eye Antibodies	>1 publications with H2AFV and Eye