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Phosphoserine (Biotinylated) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460005

Background: Protein phosphorylation is involved in cell signaling pathways. These cascades are mediated by two types of kinases: serine/threonine kinases which phosphorylate serine and threonine amino acid side chains and tyrosine kinases which phosphorylate… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phosphoserine (Biotinylated), Antibody
Full Product Name
Mouse anti Phosphoserine (pSER) antibody, Biotin Conjugated
Product Synonym Names
Mouse anti-pSER; Biotin Conjugated
Isotype
IgG
Clone Number
2A4
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
The Mouse IgG is purified by Protein A-Affinity chromatography according to isotyping.
Specificity
This antibody recognizes phospho-serine (phospho-Ser) only. It does not cross react with phospho-Tyrosine or phospho-Threonine.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.
The antibodies can be stored at 2°C to 8°C for three months without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
Antigen Preparation
A chemically linked phosphoserine.
Applications Tested/Suitable for anti-Phosphoserine (Biotinylated) antibody
WB: 0.1-1 ug/ml
EIA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/mL
IHC: 2-5 ug/mL
Flow cytometry: Not tested.
Molecular weight: Nan
Positive Control: Kidney Tissue
Cellular location: Cell membrane.
Optimal dilutions should be determined by researchers for the specific applications.

Western Blot (WB) of anti-Phosphoserine (Biotinylated) antibody

The cell lysatederived from EGF-stimulated A431was resolved onto 12% SDS-PAGE and immunoblotted by Mouse anti pSER (MBS460004) at 1:500 (lane A); or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed
Mouse Anti-Phosphoserine (Biotinylated) Antibody – Cat# MBS460005

Other Notes

Small volumes of anti-Phosphoserine (Biotinylated) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Phosphoserine (Biotinylated) purchase

MBS460005 is an antibody targeting Phosphoserine (Biotinylated). Also known as Mouse anti Phosphoserine (pSER) antibody, Biotin Conjugated. Reactive in Human, Mouse, Rat. Host: Mouse.
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