Mouse Anti-AP Monoclonal Antibody – Cat# MBS460261

Alkaline Phosphatase (AP) Antibody – Mouse Monoclonal

Name: AP Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS460261
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460261

Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues… More details

$350 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alkaline Phosphatase (AP), Monoclonal Antibody

Full Product Name

Mouse anti Alkaline Phosphatase (AP) Monoclonal Antibody

Product Gene Name

anti-AP antibody

Product Synonym Names

alkaline phosphatase, placental

Clonality

Monoclonal

Isotype

IgG1

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.

Purity/Purification

Purified by Protein A-Affinity Chromatography according to Isotyping

Specificity

This antibody recognizes human alkaline phosphatase.

Preparation and Storage

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Molecular Weight

52,198 Da

3D Structure

ModBase 3D Structure for P83456

Preparation

Alkaline phosphatase derived from bovine intestine.

Applications Tested/Suitable for anti-AP antibody

ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-AP antibody

ELISA: 0.01-0.1 ug/ml
Flow Cytometry: 5-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml

Product References and Citations for anti-AP antibody

Manoil, C, et al. Alkaline phosphatase fusions: sensors of subcellular location. J. Bacteriol., 172: 515-8 (1990)

Other Notes

Small volumes of anti-AP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AP purchase

MBS460261 is an antibody targeting AP. Also known as Mouse anti Alkaline Phosphatase (AP) Monoclonal Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for AP. It may not necessarily be applicable to this product.

NCBI GI #

41017428

NCBI GeneID

250

NCBI Accession #

P83456.1

UniProt Primary Accession #

P83456

UniProt Related Accession #

P05187

NCBI Official Full Name

Alkaline phosphatase

NCBI Official Synonym Full Names

alkaline phosphatase, placental

NCBI Official Symbol

ALPP

NCBI Official Synonym Symbols

ALP; PALP; PLAP; PLAP-1

UniProt Protein Name

Alkaline phosphatase

Protein Family

Antimicrobial protein

UniProt Gene Name

UniProt Synonym Gene Names

NCBI Protein Information

alkaline phosphatase, placental type

NCBI Summary for AP

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Research Articles on AP

1. Concentrations of PLAP were elevated in gingival crevicular fluid of patients with pre-eclampsia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AP antibody

Reference Product	PubMed Publications
FLNA antibody	>12 publications with AP and FLNA