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SOX-6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460382

SOX6 gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. SOX6… More details

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Product Overview

Online Datasheet in PDF
Product Name
SOX-6, Polyclonal Antibody
Full Product Name
Rabbit anti SOX-6 Polyclonal Antibody
Product Gene Name
Product Synonym Names
SRY (sex determining region Y)-box 6
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
This affinity purified antibody is supplied in sterile phosphate-buffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Specificity
This antibody recognizes ~94 kDa of SOX6 protein. It cross-reacts to human, mouse, rat . The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.
The antibodies can be stored at 2°C to 8°C for three months without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
Molecular Weight
~94 kDa
OMIM
AF309034 mRNA
3D Structure
ModBase 3D Structure for P35712
Antigen Preparation
A synthetic peptide corresponding to the c-terminus (from 720AA-755aa) of human sox6 protein.
This sequence is identical in human, mouse, rat, bovine, canis and chicken species.
Positive Control
Mouse embryos
Cellular Location
Nuclear
Applications Tested/Suitable for anti-SOX-6 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SOX-6 antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml

Optimal dilutions should be determined by researchers for the specific applications.

Western Blot (WB) of anti-SOX-6 antibody

The whole tissue lysate derived from Mouse embryos was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti –SOX6 (MBS460382) antibody at 1:500. An immunoreactive band around ~94 kDa was observed.
Rabbit Anti-SOX-6 Polyclonal Antibody – Cat# MBS460382

Product References and Citations for anti-SOX-6 antibody

Wilmore, H.P., Smith, M.J., Wilcox, S.A., Bell, K.M. and Sinclair, A.H. SOX14 is a candidate gene for limb defects associated with BPES and Mobius syndrome. Hum. Genet. 106 (3), 269-276 (2000)

Other Notes

Small volumes of anti-SOX-6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOX-6 purchase

MBS460382 is an antibody targeting SOX-6. Also known as Rabbit anti SOX-6 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX-6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
SOX-6, partial
NCBI Official Synonym Full Names
SRY-box 6
NCBI Official Symbol
SOX6
NCBI Official Synonym Symbols
SOXD; HSSOX6
UniProt Protein Name
Transcription factor SOX-6
Protein Family
UniProt Gene Name
SOX6
NCBI Protein Information
transcription factor SOX-6

NCBI Summary for SOX-6

This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
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UniProt Comments for SOX-6

SOX6: Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of chondrocyte differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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