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TIMP-4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460577

Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMPs comprise a family of four members: TIMP1, 2, 3, 4. TIMP-1 and TIMP-2 have similar properties, while TIMP-3 and… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TIMP-4, Polyclonal Antibody
Full Product Name
Rabbit anti TIMP-4 Polyclonal Antibody
Product Gene Name
Product Synonym Names
TIMP metallopeptidase inhibitor 4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Bovine
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
Purified by Epitope Affinity Purification
Specificity
This antibody recognizes ~25 kDa of human TIMP-4 protein. This antibody reacts with human, rat, mouse. The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2 degree C - 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
25.0
Preparation
A synthetic peptide corresponding to C-term of human TIMP-4. This sequence is identical among human, rat, mouse, chicken, dog and bovine.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-TIMP-4 antibody
ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TIMP-4 antibody
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml

Western Blot (WB) of anti-TIMP-4 antibody

Western Blot: The cell lysate derived from astrocytoma (SW-1783) was resolved onto 12% SDS-PAGE, transferred onto NC membrane, and immunoprobed by Rabbit anti-TIMP-4 (MBS460577) at 1:500. An immunoreactive band is observed around ~25 kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).
Rabbit Anti-TIMP-4 Polyclonal Antibody – Cat# MBS460577

Product References and Citations for anti-TIMP-4 antibody

Rorive, S., et al. TIMP-4 and CD63: new prognostic biomarkers in human astrocytomas. Mod. Pathol. 23 (10), 1418-1428 (2010).

Other Notes

Small volumes of anti-TIMP-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIMP-4 purchase

MBS460577 is an antibody targeting TIMP-4. Also known as Rabbit anti TIMP-4 Polyclonal Antibody. Reactive in Human, Rat, Mouse, Bovine. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMP-4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
TPA: metalloproteinase inhibitor 4
NCBI Official Symbol
TIMP4
UniProt Protein Name
Metalloproteinase inhibitor 4
UniProt Synonym Protein Names
Tissue inhibitor of metalloproteinases 4; TIMP-4
UniProt Gene Name
TIMP4
UniProt Synonym Gene Names
TIMP-4
NCBI Protein Information
metalloproteinase inhibitor 4

UniProt Comments for TIMP-4

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TIMP-4 antibody

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