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p53 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460641

P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberration. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p53, Monoclonal Antibody
Full Product Name
Mouse Anti p53 Monoclonal Antibody
Product Gene Name
Product Synonym Names
Tumor protein p53; TP53; tumor suppressor phosphoprotein p53; Mouse anti-P53 Monoclonal Antibody; Mouse anti-P53
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
S20
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
Purified by Isotyping-specific Affinity Purification.
Specificity
This antibody recognizes human P53 protein. The other species was not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
~53 kDa
Antigen Preparation
The recombinant protein of human P53 protein.
Positive Control
Breast and colon carcinomas
Cellular Location
Nuclear
Applications Tested/Suitable for anti-p53 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-p53 antibody
WB: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
IHC: 2-5 ug/ml

Immunohistochemistry (IHC) of anti-p53 antibody

IHC: The human breast cancer
tissue stained with Mouse anti
P53 monoclonal antibody
at 1:500. (Note:
formalin-fixed, paraffin-embedded
sections need 15 minutes heatinduced epitope retrieval in 10
mM citrate buffer, pH 6.0, and 30
minutes incubation at room
temperature with the primary
antibody.
Mouse Anti-p53 Monoclonal Antibody – Cat# MBS460641

Product References and Citations for anti-p53 antibody

Zakut-Houri,R., Bienz-Tadmor,B., Givol,D. and Oren,M. Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4 (5), 1251-1255 (1985).

Other Notes

Small volumes of anti-p53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p53 purchase

MBS460641 is an antibody targeting p53. Also known as Mouse Anti p53 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for p53. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Related Accession #
NCBI Official Full Name
P53
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; BMFS5; TRP53
NCBI Protein Information
cellular tumor antigen p53

NCBI Summary for p53

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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