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Heat Shock Factor 1 (HSF1) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460642

Heat-shock factor 1 (HSF1) is the master transcriptional regulator to respond to the cellular stress signals, such as heat, heavy metals, and oxidative reagents and a wide variety of other stressors. It binds to heat shock response elements (HSEs) in the… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Heat Shock Factor 1 (HSF1), Monoclonal Antibody
Full Product Name
Rat Anti Heat Shock Factor 1 Monoclonal Antibody
Product Gene Name
Product Synonym Names
Rat anti HSF1 Monoclonal Antibody; Heat shock factor protein 1; Heat Shock Factor 1 (HSF1); Heat shock transcription factor (HSTF1); Rat anti-HSF1
Clonality
Monoclonal
Isotype
IgG1k
Host
Rat
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
Purified by Affinity Chromatography according to the isotyping.
Specificity
This antibody recognizes GST-fusion proteins.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
~70 kDa
Antigen Preparation
A recombinant mouse HSF1 (1-503aa) protein.
Positive Control
LS174T cells. Breast carcinoma.
Cellular Location
Nuclear
Applications Tested/Suitable for anti-HSF1 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-HSF1 antibody
WB: 0.5-2ug/ml
ELISA: 0.01-0.1 ug/ml
IP: 2-5 ug/ml
IHC: 2-5 ug/ml

Western Blot (WB) of anti-HSF1 antibody

Western Blot: The cell lysate
was derived from MCF7 was
resolved onto 7.5% SDSPAGE, transferred onto NC
membrane, and
immunoprobed by Rat anti
HSF1 at
1:500 dilution. A major
immunoreactive band is
observed around 70kDa.
Rat Anti-HSF1 Monoclonal Antibody – Cat# MBS460642

Immunohistochemistry (IHC) of anti-HSF1 antibody

IHC: Human breast
carcinoma tissue stained
with Rat anti HSF1 antibody,
at 1:100 for
10 min @ RT. Staining of
formalin-fixed tissue
requires boiling tissue
sections in 10 mM Citrate
Buffer, pH 6.0 for 10 min
followed by cooling at RT for
20 min.
Rat Anti-HSF1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS460642

Product References and Citations for anti-HSF1 antibody

Nunes,S.L. and Calderwood,S.K. Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells. Biochem. Biophys. Res. Commun. 213 (1), 1-6 (1995).

Other Notes

Small volumes of anti-HSF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSF1 purchase

MBS460642 is an antibody targeting HSF1. Also known as Rat Anti Heat Shock Factor 1 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for HSF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Related Accession #
NCBI Official Full Name
heat shock factor 1
NCBI Official Synonym Full Names
heat shock transcription factor 1
NCBI Official Symbol
HSF1
NCBI Official Synonym Symbols
HSTF1
NCBI Protein Information
heat shock factor protein 1

NCBI Summary for HSF1

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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