• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

15-LOX-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460724

Lipoxygenases constitute a family of enzymes which are implicated in a variety of inflammatory disorders including asthma. The 15-lipoxygenase has been identified as the major route of arachidonic acid metabolism in human lung, airway epithelial cells,… More details

$335 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
15-Lox-1, Polyclonal Antibody
Full Product Name
Rabbit Anti 15-Lox-1 Polyclonal Antibody
Product Gene Name
Product Synonym Names
Rabbit anti-15-LOX1 Polyclonal Antibody; arachidonate 15-lipoxygenase; ALOX1; Rabbit anti-15-LOX-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied in sterile phosphate-buffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
Purified by Epitope Affinity Purification.
Specificity
This antibody recognizes ~75 kDa of 15-LOX1 protein. It reacts with human. The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 to -70 degree C. The antibodies can be stored at 2 to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
~75 kDa
Antigen Preparation
A synthetic peptide corresponding to the internal portion of human ALOX1 protein sequence.
Positive Control
HT-29 cells
Cellular Location
Cytoplasmic
Applications Tested/Suitable for anti-15-Lox-1 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-15-Lox-1 antibody
WB: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
IP: 2-5 ug/ml
IHC: 0.5-2 ug/ml

Western Blot (WB) of anti-15-Lox-1 antibody

Western Blot: The Cell lysate derived from HT-29 were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and immuno-blotted by Rabbit anti 15-LOX1 at 1:500. An immunoreactive band around ~75 kDa was observed.
Rabbit Anti-15-LOX-1 Polyclonal Antibody – Cat# MBS460724

Product References and Citations for anti-15-Lox-1 antibody

Sigal,E., Dicharry,S., Highland,E. and Finkbeiner,W.E. Cloning of human airway 15-lipoxygenase: identity to the reticulocyte enzyme and expression in epithelium. JOURNAL Am. J. Physiol. 262 (4 PT 1), L392-L398 (1992).

Other Notes

Small volumes of anti-15-Lox-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 15-Lox-1 purchase

MBS460724 is an antibody targeting 15-Lox-1. Also known as Rabbit Anti 15-Lox-1 Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for 15-Lox-1. It may not necessarily be applicable to this product.

NCBI GeneID

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000