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Envelope Antigen (HCV) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460740

MBS460740 is an antibody targeting HCV Envelope Antigen. Also known as Mouse Anti HCV Envelope Antigen Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HCV Envelope Antigen, Monoclonal Antibody
Full Product Name
Mouse Anti HCV Envelope Antigen Monoclonal Antibody
Product Synonym Names
Hepatitis C Virus; HCVgp1; Mouse anti-HCV NS3/NS4
Clonality
Monoclonal
Isotype
IgG1.kappa
Clone Number
ABM111
Host
Mouse
Species Reactivity
Human
Purity/Purification
Protein A affinity purification eluted according to the isotyping IgG1.kappa.
Specificity
HCV NS3/NS4 antigen
Affinicity Constant
2x109 L/Mol
Immunogen
Recombinant HCV NS3, NS4 antigen
Lymphocyte Strain
Babl/c mouse
Myeloma
P3x63 Ag 8.653
Immunization Procedure
100ul of emulsion, mixed with equal volume of recombinant HCV solution and Freund complete adjuvant is injected by using the intracutaneous injection method to each balb/c mouse, followed by boost-inject every 2 to 3 weeks with emulsion; the mice of high titer of antibody yield will be chosen for the fusion procedure.
Fusion Procedure
Fusion was performed by addition of chemical fusion reagent PEG to the mixture of spleen cells and myeloma cells at a ratio 5:1; Seeding the cell suspension to 96 well cell culture plates and selected according to principle of HAT drug blocking the de novo synthesis of nucleotides.
Screening Method
EIA method is used for clone screening, in which HCV NS3&NS4 antigen is labeled with HRP for the screening of positive clones.
Target Antigen
HCV NS3/NS4: Purity>90%
Hybridoma Cloning History
The clone was screened from a total of four 96 well cell culture plate and was subcloned twice by using EIA method. Both supernatant and purified IgG from ascites were evaluated by the method of EIA. Further evaluation was accomplished by applying purified IgG to the EIA test against normal human sera and HCV positive sera specimens; no cross reaction was found with HBeAg, HBsAg, HBcAg, AFP.
Applications Tested/Suitable for anti-HCV Envelope Antigen antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Product References and Citations for anti-HCV Envelope Antigen antibody

Masahiko Kaito, et al. Hepatitis C virus particle detected by immunoelectron microscopic study. Journal of General Virology. (1994), 75, 1735-1760. Saleh MG, Pereira LM, Tibbs CJ, Ziu M, Al-Fituri MO, Williams R, McFarlane IG. High prevalence of hepatitis C virus in the normal Libyan population. Trans R Soc Trop Med Hyg (1994), 88(3): 292-4.

Other Notes

Small volumes of anti-HCV Envelope Antigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HCV Envelope Antigen. It may not necessarily be applicable to this product.

NCBI GeneID

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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