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HIV Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460743

MBS460743 is an antibody targeting HIV. Also known as Mouse Anti HIV p24 Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV p24, Monoclonal Antibody
Full Product Name
Mouse Anti HIV p24 Monoclonal Antibody
Product Gene Name
anti-HIV antibody
Product Synonym Names
Mouse anti-HIV p24 Monoclonal Antibody; HIV p24; Gag; Mouse anti-HIV p24
Clonality
Monoclonal
Isotype
IgG1.kappa
Clone Number
ABM026
Host
Mouse
Species Reactivity
Human
Purity/Purification
Protein A affinity purification eluted according to the isotyping IgG1.kappa.
Specificity
HIV p24
Affinicity Constant
2x109 L/Mol
Immunogen
HIV1 lysate
Lymphocyte Strain
Babl/c mouse
Myeloma
P3x63 Ag 8.653
Immunization Procedure
100ul of emulsion, mixed with equal volume of HIV1 lysate solution and Freund complete adjuvant was injected by using the intracutaneous injection method to each balb/c mouse, followed by boost-inject every 2 to 3 weeks with emulsion; the mice with high titer was chosen for the fusion procedure.
Fusion Procedure
Fusion was performed by addition of chemical fusion reagent PEG to the mixture of spleen cells and myeloma cells at a ratio 5:1; Seeding the cell suspension to 96 well cell culture plates and selected according to principle of HAT drug blocking the de novo synthesis of nucleotides.
Screening Method
EIA method is used for clone screening, in which recombinant HIV p24 conjugated for the screening of positive clones.
Target Antigen
HIV p24 Purity>90%
Hybridoma Cloning History
The clone was screened from a total of four 96 well cell culture plate and was subcloned twice by using EIA method. Both supernatant and purified IgG from ascites were evaluated by the method of EIA. Further evaluation was accomplished by applying purified IgG to the EIA test against normal human sera and HIV p24 positive sera specimens; no cross reaction was found with HBeAg, HBsAg, HBcAg, and HCV.
Applications Tested/Suitable for anti-HIV antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Radioimmunoassay (RIA)

Product References and Citations for anti-HIV antibody

Forster SM, et al. Decline of anti-p24 antibody precedes antigenaemia as correlate of prognosis in HIV-1 infection. AIDS. 1(4):235-40, 1987. Lane DP and EB Lane, A rapid antibody assay system for screening hybridoma cultures, J. Immunol. Methods, 47: 303-307, 1981.

Other Notes

Small volumes of anti-HIV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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