Mouse Anti-SP1 Monoclonal Antibody – Cat# MBS460767

IHC: Human Tonsil tissue
stained with Anti-SP1
antibody, at
1:50 for 10 min @ RT.
Staining of formalin-fixed
tissue requires boiling tissue
sections in 10 mM Citrate
Buffer, pH 6.0 for 10 min
followed by cooling at RT for
20 min.

SP1 Antibody – Mouse Monoclonal

Name: SP1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS460767
Price: 390 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460767

Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. More details

$390 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SP1, Monoclonal Antibody

Full Product Name

Mouse Anti SP1 Monoclonal Antibody

Product Gene Name

anti-SP1 antibody

Product Synonym Names

Sp1 transcription factor; Mouse anti-SP1

Clonality

Monoclonal

Isotype

IgG1 kappa

Clone Number

15H09

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.

Purity/Purification

Purified by Protein A Affinity Purification.

Specificity

This antibody recognizes ~90 kDa of human SP1 protein. The other species are not tested.

Preparation and Storage

The antibodies are stable for 12 months from date of receipt when stored at -20 to -70 degree C. The antibodies can be stored at 2 to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Molecular Weight

~90 kDa

Antigen Preparation

A recombinant protein derived from 150-200 aa of human SP1.

Positive Control

Hela Cell

Cellular Location

Nuclear

Applications Tested/Suitable for anti-SP1 antibody

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Application Notes for anti-SP1 antibody

WB: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
IP: 2-5 ug/ml
FC/FACS: 5-10 ug/ml

Product References and Citations for anti-SP1 antibody

Wei-Guo Zhu; et al. Methylation of Adjacent CpG Sites Affects Sp1/Sp3 Binding and Activity in the p21Cip1 Promoter. Molecular and Cellular Biology, June 2003, p. 4056-4065, Vol. 23, No. 12.

Other Notes

Small volumes of anti-SP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SP1 purchase

MBS460767 is an antibody targeting SP1. Also known as Mouse Anti SP1 Monoclonal Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SP1. It may not necessarily be applicable to this product.

NCBI GI #

959012913

NCBI GeneID

6667

UniProt Related Accession #

P08047

NCBI Official Full Name

SP1, partial

NCBI Official Synonym Full Names

Sp1 transcription factor

NCBI Official Symbol

SP1

Protein Family

E3 ubiquitin-protein ligase

NCBI Protein Information

transcription factor Sp1

NCBI Summary for SP1

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Research Articles on SP1

1. Casp3 cleavage of transcription factor Sp1 enhances apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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