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CD8 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460777

Background: The CD8 antigen is a cell surface glycoprotein found on majority of cytotoxic T lymphocytes, thymocytes and some peripheral blood T cells. It mediates efficient cell-cell interactions within the immune system. CD8 antigen acts as a coreceptor with… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD8, Monoclonal Antibody
Full Product Name
Rat anti CD8 (Ms) Monoclonal Antibody
Product Gene Name
Product Synonym Names
Cluster of Differentiation 8
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
5H10
Host
Rat
Species Reactivity
Mouse
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
Specificity
This antibody recognizes mouse CD8 protein. The other species are not tested.
Preparation and Storage
The antibodies are stable for 24 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles
Molecular Weight
34.0
Antigen Preparation
A recombinant protein derived from extracellular domain (22-182aa) was used as an immunogen
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-CD8 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry-paraffin (IHC-p), Flow Cytometry (FC)
Application Notes for anti-CD8 antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-10 ug/ml
Flow cytometry: 0.5-5 ug/106 cells
Optimal dilutions should be determined by the researcher for specific applications.

Immunohistochemistry (IHC) of anti-CD8 antibody

Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti- CD8 (Clone 5H10) (Cat# MBS460777) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Rat Anti-CD8 Monoclonal Antibody – Cat# MBS460777

Other Notes

Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 purchase

MBS460777 is an antibody targeting CD8. Also known as Rat anti CD8 (Ms) Monoclonal Antibody. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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