Steroidogenic Factor 1 (SF1) ELISA Kit for Mouse

Name: Mouse SF1 ELISA Kit; Steroidogenic Factor 1; MyBioSource
Brand: MyBioSource
SKU: MBS046090
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS046090

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of SF1 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Steroidogenic Factor 1 (SF1), ELISA Kit

Full Product Name

Mouse Steroidogenic Factor 1 ELISA Kit

Product Gene Name

SF1 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich (Quantitative)

Detection Range

3.12 ng/ml - 100 ng/ml.

Sensitivity

1.0 ng/ml.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

51,636 Da

Chromosome Location

Chromosome: 9; NC_000009.11 (127243515..127269699, complement). Location: 9q33

OMIM

184757

3D Structure

ModBase 3D Structure for Q13285

Other Notes

Small volumes of SF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SF1 purchase

MBS046090 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Steroidogenic Factor 1 (SF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SF1. The ELISA analytical biochemical technique of the MBS046090 kit is based on SF1 antibody-SF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SF1. It may not necessarily be applicable to this product.

NCBI GI #

20070193

NCBI GeneID

2516

NCBI Accession #

NP_004950.2

UniProt Primary Accession #

Q13285

UniProt Secondary Accession #

O15196; Q5T6F5

UniProt Related Accession #

Q13285

GenBank Nucleotide #

NM_004959.4

NCBI Official Full Name

steroidogenic factor 1

NCBI Official Synonym Full Names

nuclear receptor subfamily 5, group A, member 1

NCBI Official Symbol

NR5A1

NCBI Official Synonym Symbols

ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3

UniProt Protein Name

Steroidogenic factor 1

UniProt Synonym Protein Names

Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP

Protein Family

Splicing factor

UniProt Gene Name

NR5A1

UniProt Synonym Gene Names

AD4BP; FTZF1; SF1; SF-1; STF-1

UniProt Entry Name

STF1_HUMAN

NCBI Protein Information

steroidogenic factor 1; STF-1; nuclear receptor AdBP4; steroidogenic factor-1; adrenal 4 binding protein; adrenal 4-binding protein; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroidogenic factor 1 nuclear receptor

NCBI Summary for SF1

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]

UniProt Comments for SF1

STF-1: a nuclear hormone receptor of the NR5 subfamily. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; double-stranded DNA binding; transcription coactivator activity; phospholipid binding; steroid hormone receptor activity; chromatin binding

Biological Process: tissue development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; adrenal gland development; primary sex determination; male gonad development; multicellular organismal aging; regulation of steroid biosynthetic process; cell-cell signaling; luteinization; hormone metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; maintenance of protein localization in nucleus; cell differentiation

Disease: Premature Ovarian Failure 7; 46,xy Sex Reversal 3; Spermatogenic Failure 8

Research Articles on SF1

1. genetic association studies in population in Europe/North Africa: Data exclude NR5A1 mutations (missense mutations in 3 subjects) as a major genetic cause of primary ovarian insufficiency.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SF1 elisa kit

Reference Product	PubMed Publications
NR0B1 elisa kit	>73 publications with SF1 and NR0B1
SP1 elisa kit	>18 publications with SF1 and SP1
NR4A1 elisa kit	>15 publications with SF1 and NR4A1
FOXL2 elisa kit	>14 publications with SF1 and FOXL2
TRERF1 elisa kit	>5 publications with SF1 and TRERF1
ESR2 elisa kit	>3 publications with SF1 and ESR2
ESR1 elisa kit	>3 publications with SF1 and ESR1
NR0B2 elisa kit	>2 publications with SF1 and NR0B2

Pathways associated with SF1 elisa kit

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Nuclear Receptor Transcription Pathway antibodies	Nuclear Receptor Transcription Pathway Diagram
Nuclear Receptors Pathway antibodies	Nuclear Receptors Pathway Diagram
Ovarian Infertility Genes Pathway antibodies	Ovarian Infertility Genes Pathway Diagram
Wnt Signaling Pathway NetPath antibodies	Wnt Signaling Pathway NetPath Diagram

Diseases associated with SF1 elisa kit

Disease Name	Pubmed Publications
Disorders of Sex Development Antibodies	>66 publications with SF1 and Disorders of Sex Development
Urogenital Abnormalities Antibodies	>61 publications with SF1 and Urogenital Abnormalities
Neoplasms Antibodies	>59 publications with SF1 and Neoplasms
Adrenal Insufficiency Antibodies	>43 publications with SF1 and Adrenal Insufficiency
Carcinoma Antibodies	>33 publications with SF1 and Carcinoma
46,XY Sex Reversal 3 Antibodies	>32 publications with SF1 and 46,XY Sex Reversal 3
Gonadal Dysgenesis, 46,XY Antibodies	>31 publications with SF1 and Gonadal Dysgenesis, 46,XY
Ovarian Diseases Antibodies	>27 publications with SF1 and Ovarian Diseases
Primary Ovarian Insufficiency Antibodies	>20 publications with SF1 and Primary Ovarian Insufficiency
Genital Diseases, Male Antibodies	>20 publications with SF1 and Genital Diseases, Male

Organs/Tissues associated with SF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Adrenal Gland Antibodies	>130 publications with SF1 and Adrenal Gland
Testis Antibodies	>114 publications with SF1 and Testis
Ovary Antibodies	>113 publications with SF1 and Ovary
Brain Antibodies	>59 publications with SF1 and Brain
Liver Antibodies	>49 publications with SF1 and Liver
Spleen Antibodies	>13 publications with SF1 and Spleen
Thymus Antibodies	>4 publications with SF1 and Thymus
Connective Tissue Antibodies	>2 publications with SF1 and Connective Tissue
Uncharacterized Tissue Antibodies	>1 publications with SF1 and Uncharacterized Tissue