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AKT1/PKBa (pS473) Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462005

Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)]; Akt is activated by… More details

$335 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AKT1/PKBa (pS473), Antibody
Full Product Name
Rabbit anti Phospho- AKT1/PKBalpha (pSER473) antibody
Product Gene Name
anti-AKT1/PKBa (pS473) antibody
Product Synonym Names
Rabbit anti-AKT1 (pS473)
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Site-directed adsorption and epitope affinity purification. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Concentration
Size: 100 ug/200 ul (lot specific)
Specificity
The motif corresponding to the major autophosphorylation site of the human AKT1 kinase protein. This antibody does not cross react to the non-phosphorylated AKT1 or with other unrelated phosphorylated serine. Ser473 corresponds to QFSYS in human, rat, mouse, chicken and canis of AKT/PKB proteins
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
~60 kDa
Antigen Preparation
A synthetic peptide containing the phosphorylated Ser473 motif of human AKT1.
Positive Control
PDGF-Treated 3T3 cell lysate.
Cellular Location
Cell Cytoplasmic
Applications Tested/Suitable for anti-AKT1/PKBa (pS473) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-AKT1/PKBa (pS473) antibody
Western Blot: 0.1-0.2 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1-2 ug/ml

Testing Data of anti-AKT1/PKBa (pS473) antibody

Rabbit Anti-AKT1/PKBa (pS473) Antibody – Cat# MBS462005

Other Notes

Small volumes of anti-AKT1/PKBa (pS473) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKT1/PKBa (pS473) purchase

MBS462005 is an antibody targeting AKT1/PKBa (pS473). Also known as Rabbit anti Phospho- AKT1/PKBalpha (pSER473) antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.
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