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Ki-67 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462066

Ki-67, a proliferation marker is a nuclear protein that is associated with and may be necessary for cellular proliferation. It can be used as a biomarker with Bcl-2 (an apoptosis inhibitor), P53 and Pax2 for immunohistostaining in carcinomas identification.… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ki-67, Antibody
Full Product Name
Rabbit anti ki-67 antibody
Product Gene Name
Product Synonym Names
Rabbit anti-Ki-67
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
The Rabbit IgG is purified by Epitope affinity purification
Concentration
Size: 100 ug/200 ul (lot specific)
Specificity
This antibody recognizes high molecular weight of Ki-67 protein. It reacts with human, rat and mouse. The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
345 & 395 kDa
3D Structure
ModBase 3D Structure for Q61769
Antigen Preparation
A synthetic peptide derived from human ki-67 protein.
Positive Control
Breast carcinomas and lymph node
Cellular Location
Nuclei
Applications Tested/Suitable for anti-Ki-67 antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-Ki-67 antibody
Western Blot: 0.5-2 ug/ml
ELISA: 0.1-1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Flow Cytometry: Not Tested

Testing Data of anti-Ki-67 antibody

Rabbit Anti-Ki-67 Antibody – Cat# MBS462066

Product References and Citations for anti-Ki-67 antibody

Mourtzikou A, Kosmas K, Marouga A, Stamouli M, Pouliakis A, Karakitsos P. The use of an immunocytochemical double-labeling staining can display the distribution of Bcl-2/Ki-67 cells in endometrial adenocarcinomas as well as in normal endometrium. Clin Lab. 2012;58(1-2):133-44.

Other Notes

Small volumes of anti-Ki-67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ki-67 purchase

MBS462066 is an antibody targeting Ki-67. Also known as Rabbit anti ki-67 antibody. Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ki-67. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
NCBI Official Full Name
Ki-67
UniProt Protein Name
Ki-67 protein
UniProt Gene Name
Mki67
UniProt Synonym Gene Names
Ki-67
UniProt Entry Name
Q61769_MOUSE

UniProt Comments for Ki-67

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Cell cycle regulation; Nucleolus

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding

Biological Process: meiosis; cell proliferation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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