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PAK4 (pS474) Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462102

p21-activated protein kinase (PAK) serine/threonine kinases are important effectors of Rho family GTPases and have been implicated in the regulation of cell morphology and motility, as well as in cell transformation. The phosphorylation of serine 474 as the… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PAK4 (pS474), Antibody
Full Product Name
Rabbit anti Phospho-PAK4 (pSer474)
Product Gene Name
anti-PAK4 (pS474) antibody
Product Synonym Names
Rabbit anti-PAK4 (pS474); p21-activated kinase 4
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Bovine, Chicken, Canine
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The Rabbit IgG is purified by phosphospecific-Epitope Affinity Purification.
Concentration
Size: 100 ug/200 ul (lot specific)
Specificity
This antibody recognizes the phosphorylated peptide only. It recognizes PAK4 protein at a phosphorylation site of Serine 474 (~68kDa), or serine 602 of PAK5 (~90kDa) or Serine 560 of PAK6 (~80 kDa). It does not cross react to other PAK1, PAK2 or PAK3. This antibody reacts with human, rat and mouse. The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
68 kDa
Antigen Preparation
A synthetic peptide surrounding the epitope RKSLV-with a phosphorylation at Serine 474 of human PAK4. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
Positive Control
HEK293
Applications Tested/Suitable for anti-PAK4 (pS474) antibody
ELISA, Western Blot, Immunoprecipitation
Application Notes for anti-PAK4 (pS474) antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml

Testing Data of anti-PAK4 (pS474) antibody

Rabbit Anti-PAK4 (pS474) Antibody – Cat# MBS462102

Product References and Citations for anti-PAK4 (pS474) antibody

Marinella G. Callow et al. Requirement for PAK4 in the Anchorage-independent Growth of Human Cancer Cell Lines. (2002) J. Biol. Chem. 277 (1), 550-558.

Other Notes

Small volumes of anti-PAK4 (pS474) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAK4 (pS474) purchase

MBS462102 is an antibody targeting PAK4 (pS474). Also known as Rabbit anti Phospho-PAK4 (pSer474). Reactive in Human, Rat, Mouse, Bovine, Chicken, Canine. Host: Rabbit.
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