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JNK/SAPK1 (pT183/pY185) Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462109

c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK). There are three isoforms, JNK1 (~49 kDa), JNK2 (~55 kDa), and JNK3 (~54 kDa). JNK is dually phosphorylated at Thr 183 and Tyr 185 by protein kinases MKK4 and MKK7. The… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
JNK/SAPK1 (pT183/pY185), Antibody
Full Product Name
Rabbit anti Phospho- JNK/SAPK (pThr183/pTyr185)
Product Synonym Names
Rabbit anti-Phospho-JNK/SAPK (pT183/pY185); c-Jun N-terminal Kinase (JNK); Stress Activated Protein Kinase (SAPK); mitogen-activated protein kinase 8/9 (MAPK8; MAPK9)
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken, Bovine Canine
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Concentration
Size: 100 ug/200 ul (lot specific)
Specificity
This antibody recognizes human JNK protein. It also reacts with rat and mouse JNK protein. The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
49, 55 kDa
Antigen Preparation
A synthetic peptide surrounding to the epitope -TPY- with dual phosphorylation sites at Thr183 and Tyr185 of human JNK/SAPK.
Positive Control
UV treated-HEK293
Cellular Location
Cytoplasmic
Applications Tested/Suitable for anti-JNK/SAPK1 (pT183/pY185) antibody
ELISA, Western Blot, Immunohistochemistry
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:50 ug/ml

Product References and Citations for anti-JNK/SAPK1 (pT183/pY185) antibody

Yogev,O., et al. DNA damage-dependent translocation of B23 and p19 ARF is regulated by the Jun N-terminal kinase pathway. Cancer Res. 68 (5), 1398-1406 (2008)

Other Notes

Small volumes of anti-JNK/SAPK1 (pT183/pY185) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JNK/SAPK1 (pT183/pY185) purchase

MBS462109 is an antibody targeting JNK/SAPK1 (pT183/pY185). Also known as Rabbit anti Phospho- JNK/SAPK (pThr183/pTyr185). Reactive in Human, Rat, Mouse, Chicken, Bovine Canine. Host: Rabbit.
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