Mouse Anti-CXCR3 Monoclonal Antibody – Cat# MBS462161

Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti-CXCR3 at 1:500 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min

CXCR3 Antibody – Mouse Monoclonal

Name: CXCR3 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS462161
Price: 355 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462161

Chemokine receptors are seven-transmembrane domain G-protein coupled receptors that mediate the biological activities of chemokines. Most of these receptors exhibit promiscuous binding properties whereby several different chemokines signal through the same… More details

$355 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CXCR3, Monoclonal Antibody

Full Product Name

Mouse anti CXCR3 Monoclonal antibody

Product Gene Name

anti-CXCR3 antibody

Product Synonym Names

Mouse anti-CXCR3; Chemokine CXC Motif receptor 3; CD183; GPR9; IP10R; MIGR

Clonality

Monoclonal

Isotype

IgG1, k

Clone Number

1C6

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Form/Format

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH 7.2) containing antibody stabilizer

Purity/Purification

The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping

Specificity

This antibody recognizes surface expressed CXCR3, and/or PHA or anti-CD3 activated human T cells. The other species are not tested.

Preparation and Storage

The antibodies are stable for 24 months from date of receipt when stored at -20°C to -70°C.
The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity.
Avoid repeated freezing-thawing cycles.

Molecular Weight

41.0

Chromosome Location

Chromosome: X; NC_000023.10 (70835766..70838367, complement). Location: Xq13

OMIM

300574

3D Structure

ModBase 3D Structure for P49682

Antigen Preparation

A 37aa-synthetic peptide corresponding to the N-terminus of human CXCR3 conjugated to KLH.

Positive Control

Kidney Tissue

Cellular Location

Cell Membrane

Applications Tested/Suitable for anti-CXCR3 antibody

Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC)

Application Notes for anti-CXCR3 antibody

Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-10 ug/ml
Flow Cytometry: 0.5-5 ug/10⁶ cells

Optimal dilutions should be determined by researchers for the specific applications

Product References and Citations for anti-CXCR3 antibody

Qin S, et al. The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. J. Clin. Invest. 101: 746-754, 1998.

Other Notes

Small volumes of anti-CXCR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCR3 purchase

MBS462161 is an antibody targeting CXCR3. Also known as Mouse anti CXCR3 Monoclonal antibody. Reactive in Human, Mouse, Rat . Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCR3. It may not necessarily be applicable to this product.

NCBI GI #

218563730

NCBI GeneID

2833

NCBI Accession #

NP_001136269.1

UniProt Primary Accession #

P49682

UniProt Secondary Accession #

O15185; Q7Z710; Q9P2T4; Q9P2T5

UniProt Related Accession #

P49682

GenBank Nucleotide #

NM_001142797.1

NCBI Official Full Name

C-X-C chemokine receptor type 3 isoform 2

NCBI Official Synonym Full Names

chemokine (C-X-C motif) receptor 3

NCBI Official Symbol

CXCR3

NCBI Official Synonym Symbols

GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R

UniProt Protein Name

C-X-C chemokine receptor type 3

UniProt Synonym Protein Names

CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor

Protein Family

C-X-C chemokine receptor

UniProt Gene Name

CXCR3

UniProt Synonym Gene Names

GPR9

UniProt Entry Name

CXCR3_HUMAN

NCBI Protein Information

C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; OTTHUMP00000032170; OTTHUMP00000070257; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor

NCBI Summary for CXCR3

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq]

UniProt Comments for CXCR3

Function: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Ref.2 Ref.8Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis. Ref.2 Ref.8Isoform 3 mediates the activity of CXCL11. Ref.2 Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Ref.2

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

Sequence caution: The sequence CAB02143.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on CXCR3

1. Results demonstrated the expression of CXCR3 as well as of two of its known variants, CXCR3-B and CXCR3-alt, in ex vivo activated T lymphocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CXCR3 antibody

Reference Product	PubMed Publications
CXCL10 antibody	>611 publications with CXCR3 and CXCL10
CXCL9 antibody	>351 publications with CXCR3 and CXCL9
CXCL11 antibody	>228 publications with CXCR3 and CXCL11
CXCL13 antibody	>21 publications with CXCR3 and CXCL13
PF4 antibody	>11 publications with CXCR3 and PF4

Pathways associated with anti-CXCR3 antibody

Products by Pathway	Pathway Diagram
CXCR3-mediated Signaling Events Pathway antibodies	CXCR3-mediated Signaling Events Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-CXCR3 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>420 publications with CXCR3 and Inflammation
Liver Diseases Antibodies	>82 publications with CXCR3 and Liver Diseases
Carcinoma Antibodies	>72 publications with CXCR3 and Carcinoma
Fibrosis Antibodies	>61 publications with CXCR3 and Fibrosis
Kidney Diseases Antibodies	>48 publications with CXCR3 and Kidney Diseases
Adenocarcinoma Antibodies	>36 publications with CXCR3 and Adenocarcinoma
Skin Neoplasms Antibodies	>32 publications with CXCR3 and Skin Neoplasms
Dermatitis, Atopic Antibodies	>31 publications with CXCR3 and Dermatitis, Atopic
Encephalomyelitis, Autoimmune, Experimental Antibodies	>27 publications with CXCR3 and Encephalomyelitis, Autoimmune, Experimental
Neoplasm Metastasis Antibodies	>24 publications with CXCR3 and Neoplasm Metastasis

Organs/Tissues associated with anti-CXCR3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1073 publications with CXCR3 and Blood
Brain Antibodies	>103 publications with CXCR3 and Brain
Spleen Antibodies	>87 publications with CXCR3 and Spleen
Intestine Antibodies	>52 publications with CXCR3 and Intestine
Muscle Antibodies	>40 publications with CXCR3 and Muscle
Connective Tissue Antibodies	>7 publications with CXCR3 and Connective Tissue
Uncharacterized Tissue Antibodies	>1 publications with CXCR3 and Uncharacterized Tissue