• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

BIOTIN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462194

This antibody has Ab-1 has a very high affinity for biotin and is excellent for use in various amplification techniques. It recognizes both the free and protein conjugated (either soluble or cell bound) form of biotin. In some applications, localization of… More details

$315 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Biotin, Monoclonal Antibody
Full Product Name
Mouse anti Biotin Monoclonal antibody
Product Gene Name
Product Synonym Names
Mouse anti-Biotin; N/A
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
SPM375
Host
Mouse
Species Reactivity
All species
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
Specificity
This antibody recognizes free Biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
244.0
Antigen Preparation
Biotin-conjugates as an immunogen.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-Biotin antibody
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
Application Notes for anti-Biotin antibody
WB: 0.1-1 ug/ml
EIA: 0.01-0.1 ug/ml
IP: 2-5 ug/ml
IHC: 2-10 ug/mL

Optimal dilutions should be determined by researchers for the specific applications.

IHC of anti-Biotin antibody

Biotinylated STAT5 antibody was detected by MsxBiotin at 1:100, and visualized by FITC-Goat anti Ms IgG. The STAT5 is nuclear located.
Mouse Anti-BIOTIN Monoclonal Antibody – Cat# MBS462194

Product References and Citations for anti-Biotin antibody

Elisabeth D. Coene et al (2008). Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin. Methods in Molecular Biology, Volume 418. 157-170.

Other Notes

Small volumes of anti-Biotin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Biotin purchase

MBS462194 is an antibody targeting Biotin. Also known as Mouse anti Biotin Monoclonal antibody. Reactive in All species. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Biotin. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000