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GSK-3b (pY216) Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462202

Glycogen Synthase Kinase-3beta (GSK-3beta) is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase. Growth factor (such as Insulin) binds to its receptor, recruits PKB/AKT… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GSK-3b (pY216), Antibody
Full Product Name
Rabbit anti Phospho- GSK-3beta (pTyr 216)
Product Synonym Names
Rabbit anti-Phospho-GSK3b (pY216); glycogen synthase kinase 3 beta (GSK3-b)
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Bovine, Chicken
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Specificity
This antibody recognizes GSK-3beta (pY216) with a phosphorylated site at Tyrosine 216. It does not crossreact with non-phosphospecific peptide.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
46.0 kDa
Antigen Preparation
A synthetic peptide surrounding to the epitope -NVSYI- with a phosphorylation site at Tyr216 of human GSK3-beta protein. This sequence is identical among human, mouse, rat, bovine, dog and chicken species.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-GSK-3b (pY216) antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-GSK-3b (pY216) antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml

Optimal dilutions should be determined by researchers for the specific applications.

Western Blot (WB) of anti-GSK-3b (pY216) antibody

Western Blot: : The EGF stimulated A431 cell lysates (20 ug/lane) immunoblotted by Rabbit anti GSK-3beta (pY216) (Cat# MBS462202) antibody at 1:500. Observed a major immunoreactive band at molecular weight ~46kDa.
Rabbit Anti-GSK-3b (pY216) Antibody, Western Blot (WB) – Cat# MBS462202

Immunohistochemistry (IHC) of anti-GSK-3b (pY216) antibody

Immunohistochemistry: Human carcinoma (FFPE) stained with Rabbit anti-TGF at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Rabbit Anti-GSK-3b (pY216) Antibody, Immunohistochemistry (IHC) – Cat# MBS462202

Product References and Citations for anti-GSK-3b (pY216) antibody

Alan P. Kozikowski, et al. Highly Potent and Specific GSK-3beta Inhibitors That Block Tau Phosphorylation and Decrease alpha-Synuclein Protein Expression in a Cellular Model of Parkinson's Disease. ChemMedChem, 2006, 1 (2), 256-266.

Other Notes

Small volumes of anti-GSK-3b (pY216) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSK-3b (pY216) purchase

MBS462202 is an antibody targeting GSK-3b (pY216). Also known as Rabbit anti Phospho- GSK-3beta (pTyr 216). Reactive in Human, Rat, Mouse, Bovine, Chicken. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GSK-3b (pY216). It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
glycogen synthase kinase 3 beta

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GSK-3b (pY216) antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with GSK-3b (pY216) and FLNA
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