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HIS-TAG Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462204

Many oligo peptides or tag proteins are widely used as a tag to monitor the protein expression, such as DYKDDDDK, c-Myc, E-tag, His-tag, GFP, GST, and etc. These tag antibodies provide tools to localize gene products in a variety of cell types to study the… More details

$310 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
His-Tag, Polyclonal Antibody
Full Product Name
Rabbit anti His Tag Polyclonal antibody
Product Gene Name
Product Synonym Names
Rabbit anti-His tag; HHHHHH
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Tag Antibody
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The Rabbit IgG is purified by Epitope-Affinity Chromatography
Concentration
Size: 100 ug/200 ul (lot specific)
Specificity
This antibody recognizes His tagged fusion proteins. It is a tag antibody.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Antigen Preparation
A synthetic peptide of HHHHHH conjugated to a carrier protein
Positive Control
His tagged fused proteins
Applications Tested/Suitable for anti-His-Tag antibody
ELISA, Western Blot, Immunoprecipitation
Application Notes for anti-His-Tag antibody
Western Blot: 0.5- 2ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml

Testing Data of anti-His-Tag antibody

Rabbit Anti-HIS-TAG Polyclonal Antibody – Cat# MBS462204

Product References and Citations for anti-His-Tag antibody

Els Conrath, K et al (2001), J. Biol. Chem. 276: 7346-7350.

Other Notes

Small volumes of anti-His-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for His-Tag purchase

MBS462204 is an antibody targeting His-Tag. Also known as Rabbit anti His Tag Polyclonal antibody. Reactive in Tag Antibody. Host: Rabbit.
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